Project description:We wanted to identify genes regulated by the two-component signal transduction system RR/HK06 in Streptococcus pneumoniae. We used microarray in order to discover regulated genes, investigating the effects of the over-expression of RR06 in D39 (serotype 2). Keywords: Gene Regulation
Project description:Streptococcus pneumoniae is opportunistic bacteria cause’s acute otitis media (AOM) in children. It colonizes the nasopharynx in the form of biofilms, and these biofilms act as reservoir, and are vital for pneumococcal infections. The pneumococcal biofilms are regulated by LuxS/AI-2 media quorum sensing. In this study, we confirmed the role of LuxS/AI-2 for in vitro formation of biofilms, assessed the effects of the absence of LuxS/AI-2 signaling, for pneumococcal middle ear infection and identified global genes regulated by LuxS/AI-2 during formation of pneumococcal biofilms. In the cDNA-microarray analysis, 117 genes were differentially expressed in D39 luxS mutant when compared with D39 wild type. Among the 66 genes encoding putative proteins and previously characterized proteins, 60 were significantly down-regulated and 6 were significantly up-regulated. The functional annotation revealed that genes involve in DNA replication and repair, ATP synthesis, capsule biosynthesis, cell division and cell cycle, signal transduction, transcription regulation, competence, virulence, and carbohydrate metabolism were down-regulated in the absence of LuxS/AI-2.
Project description:We wanted to identify genes regulated by the two-component signal transduction system RR/HK06 in Streptococcus pneumoniae. We used microarray in order to discover regulated genes, investigating the effects of the over-expression of RR06 in TIGR4 (serotype 4). Keywords: Gene Regulation
Project description:Streptococcus pneumoniae D39 AdcR (adhesion competence repressor) is the first metal-sensing member of the MarR (multiple antibiotic resistance repressor) family to be characterized. Expression profiling of a ∆adcR strain grown in liquid culture under microaerobic conditions revealed that transcript amounts for 13 genes were up-regulated relative to the wild-type strain, among them adcR, adcCBA, encoding a high affinity ABC uptake system for zinc, and genes encoding cell-surface zinc-binding pneumococcal histidine triad (pht) and adcII (lmb, laminin binding) proteins. Down-regulated transcripts included those encoding two putative zinc-containing alcohol dehydrogenases.
Project description:By comparing the transcriptome of Streptococcus pneumoniae wild-type D39 (serotype 2) to an isogenic strain overexpressing the response regulator of Two-Component System 7 (SPD_0158), we identified the regulon of the two-component system. In total 20 and 24 genes were considered up- and downregulated, respectively. 11 of the upregulated genes encode for proteins involved in host-glycan metabolism, while 7 encode proteins involved in carbohydrate metabolism. Most downregulated genes encode competence genes, but later experiments proved this was likely due to an atefact of overexpression. The remaining genes encode genes with other functions.
Project description:A conserved two-component signal transduction system controls the response to phosphate starvation in Bifidobacterium breve UCC2003
Project description:We reported the RNA-Seq results of Staphylococcus aureus Newman wild-type, walKD119A, walKV149A and DHBP-treated wild-type strains. We found that mutations of the potential signal transduction residues of WalK attenuate the activity of walKR two-component system, whereas DHBP supplementation activates this two-component system.
