Project description:This study aimed to identify genes that were differentially expressed when D. nodosus was grown on agar media supplemented with ground hoof compared to the standard agar media. Keywords: growth response to hoof material
Project description:This study aimed to identify genes that were differentially expressed when D. nodosus was grown on agar media supplemented with ground hoof compared to the standard agar media. Bacteria were grown on EYE agar or EYE agar with 2% ground hoof agar plates for 2 days before Trizol extraction of RNA. Four biological replicates including two dye swaps were analysed. Raw data was compiled using IMAGENE. Normalisation and statistical analysis was undertaken using BASE. Statistics were performed using the Wilcoxon signed ranked test.
Project description:To determine the genes in addition to fimA regulated by sigma 54 by analysing the gene expression profiles of an rpoN mutant compared to the D. nodosus wild-type strain. Keywords: genetic modification
