Effects of chronic ingestion of soy peptide supplementation on gut microbial composition during juvenile social isolation
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ABSTRACT:
PROVIDER: PRJNA966981 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24416128_1.fastq.gz | Fastqsanger.gz | |||
| SRR24416128_2.fastq.gz | Fastqsanger.gz | |||
| SRR24416129_1.fastq.gz | Fastqsanger.gz | |||
| SRR24416129_2.fastq.gz | Fastqsanger.gz | |||
| SRR24416130_1.fastq.gz | Fastqsanger.gz |
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Project description:To investigate the effect of soy peptides on gut microial composition during juvenile social isolation, group-house (GH) and social isolation (SI) mice were fed a diet consisting of soy peptides or a control diet for 4 weeks post-weaning. We then performed microbial community analysis using data obtained from bacterial 16S rRNA gene sequencing in the fecal samples of 4 mice groups (control diet-fed GH, soy peptide-diet fed GH, control diet-fed SI, and soy peptide-diet fed SI mice).
2024-05-29 | GSE231557 | GEO
Project description:Background There are conflicting reports on the impact of soy on breast carcinogenesis. This study examines the effects of soy supplementation on breast cancer-related genes and pathways. Methods Women (n = 140) with early-stage breast cancer were randomized to soy protein supplementation (n = 70) or placebo (n = 70) for 7 to 30 days, from diagnosis until surgery. Adherence was determined by plasma isoflavones: genistein and daidzein. Gene expression changes were evaluated by NanoString inin pre- and post-treatment tumor tissue. Genome-wide expression analysis was performed on post-treatment tissue. Proliferation (Ki67) and apoptosis (Cas3) were assessed by immunohistochemistry. Results Plasma isoflavones rose in the soy group (two-sided Wilcoxon rank-sum test, P < .001) and did not change in the placebo group. In paired analysis of pre- and post-treatment samples, 21 genes (out of 202) showed altered expression (two-sided Student’s t-test, P < .05). Several genes including FANCC and UGT2A1 revealed different magnitude and direction of expression changes between the two groups (two-sided Student’s t-test, P < .05). A high-genistein signature consisting of 126 differentially expressed genes was identified from microarray analysis of tumors. This signature was characterized by overexpression (>2 fold) of cell cycle transcripts, including those which promote cell proliferation, such as FGFR2, E2F5, BUB1, CCNB2, MYBL2, CDK1, and CDC20 (P < .01). Soy intake did not result in statistically significant changes in Ki67 or Cas3. Conclusions Gene expression associated with soy intake and high plasma genistein define a signature characterized by overexpression of FGFR2 and genes that drive cell cycle and proliferation pathways. These findings raise the concerns that in a subset of women soy could adversely affect gene expression in breast cancer.
2014-07-01 | GSE58792 | GEO
Project description:Background There are conflicting reports on the impact of soy on breast carcinogenesis. This study examines the effects of soy supplementation on breast cancer-related genes and pathways. Methods Women (n = 140) with early-stage breast cancer were randomized to soy protein supplementation (n = 70) or placebo (n = 70) for 7 to 30 days, from diagnosis until surgery. Adherence was determined by plasma isoflavones: genistein and daidzein. Gene expression changes were evaluated by NanoString inin pre- and post-treatment tumor tissue. Genome-wide expression analysis was performed on post-treatment tissue. Proliferation (Ki67) and apoptosis (Cas3) were assessed by immunohistochemistry. Results Plasma isoflavones rose in the soy group (two-sided Wilcoxon rank-sum test, P < .001) and did not change in the placebo group. In paired analysis of pre- and post-treatment samples, 21 genes (out of 202) showed altered expression (two-sided Student’s t-test, P < .05). Several genes including FANCC and UGT2A1 revealed different magnitude and direction of expression changes between the two groups (two-sided Student’s t-test, P < .05). A high-genistein signature consisting of 126 differentially expressed genes was identified from microarray analysis of tumors. This signature was characterized by overexpression (>2 fold) of cell cycle transcripts, including those which promote cell proliferation, such as FGFR2, E2F5, BUB1, CCNB2, MYBL2, CDK1, and CDC20 (P < .01). Soy intake did not result in statistically significant changes in Ki67 or Cas3. Conclusions Gene expression associated with soy intake and high plasma genistein define a signature characterized by overexpression of FGFR2 and genes that drive cell cycle and proliferation pathways. These findings raise the concerns that in a subset of women soy could adversely affect gene expression in breast cancer. Genome-wide expression analysis was performed on breast cancer tissue from women following treatment with soy (n=28) or placebo (n=23).
2014-07-01 | E-GEOD-58792 | biostudies-arrayexpress
Project description:The lateral habenula (LHb) is an essential hub brain region modulating the monoamine system such as dopamine, serotonin. Hyperactivity of LHb has implications for psychiatric disorders such as depression, anxiety, and schizophrenia, which are commonly associated with social dysfunction. However, the role of LHb in social behavior has remained elusive. Here, we find that experiencing acute social isolation affects synaptic function in LHb and social behavior. After acute social isolation, long-term depression (LTD) in LHb is impaired and rescued by activating the 5-HT4 receptor (5-HT4R). Indeed, Htr4 expression in LHb is up-regulated following acute social isolation. Finally, acute social isolation enhances the social preference for familiars such as housing-mates to stranger conspecifics. Consistent with electrophysiological results, pharmacological activation of 5-HT4R in LHb restored innate social preference. These results suggest that acute social isolation influences social decisions with 5-HT4R-dependent synaptic modification in LHb.
2024-04-30 | GSE200082 | GEO
Project description:To investigate gene expression changes in Drosophila head tissues during social isolation, we performed RNA-sequencing on fruit fly head samples obtained from male flies that have been group-reared for 7 days (Grp), isolated (single-housed) for 7 days (Iso7) and isolated (single-housed) for only 1 day (Iso1). Using differential gene expression analysis, we found a group of candidate genes that are specific to chronic social isolation: they exhibited significant gene expression change in both comparisons of “Grp vs Iso1” and “Iso1 vs Iso7”.
2021-06-15 | GSE137498 | GEO
Project description:Clinical studies have revealed that social support improves the outcome of cancer patients while epidemiological studies suggest that social isolation increases the risk of death associated with several chronic diseases. However, the precise biological consequences of an unfavorable social environment have not been defined. To do so, robust, reproducible pre-clinical models are needed to study the mechanisms whereby an adverse environment impacts on gene expression and cancer biology. Because random assignment of inbred laboratory mice to well-defined social environments allows accurate and repeated measurements of behavioral and endocrine parameters, transgenic mice provide a pre-clinical framework with which to begin to determine gene-environment mechanisms. In this study, we found that female C3(1)/SV40 T-antigen mice deprived of social interaction from weaning exhibited increased expression of genes encoding key metabolic pathway enzymes in the pre-malignant mammary gland. Chronic social isolation was associated with upregulated fatty acid synthesis and glycolytic pathway gene expression - both pathways known to contribute to increased breast cancer growth. Consistent with the expression of metabolic genes, isolated mice subsequently developed significantly larger mammary gland tumors compared to group-housed mice. Endocrine evaluation confirmed that isolated mice developed a heightened corticosterone stress response compared to group-housed mice. Together, these transdisciplinary studies show for the first time that an adverse social environment is associated with altered mammary gland gene expression and tumor growth. Moreover, the identification of specific alterations in metabolic pathways favoring tumor growth suggests potential molecular biomarkers and/or targets (e.g. fatty acid synthesis) for preventive intervention in breast cancer.
2009-07-01 | GSE16110 | GEO
Project description:Individual differences in basal leukocyte gene expression profiles as a function of perceived social isolation (PSI) Gene expression profiling was carried out on peripheral blood mononuclear cell RNA samples collected from 62 adults in Year 7 of the Chicago Health Aging and Social Relations Study. Participants were assessed for PSI using the UCLA Loneliness Scale, and were also classified as chronically high in PSI (1/0) based on UCLA Loneliness Scale scores during study years 1-5. A 1-year lagged value of PSI is also included, and analyses additionally controlled for age at study entry, sex, race/ethnicity (dummy codes for Black and Hispanic), marital status, (log) household income, Body Mass Index (BMI), smoking history, heavy alcohol consumption, depressive symptoms (CESD), perceived stress (PSS), social support (SocSupp), circulating monocyte percentages, and RNA indicators of major leukocyte subsets (CD3E, CD3D, CD4, CD8A, CD19, FCGR3A/CD16, NCAM1/CD56, CD14).
2015-11-23 | E-GEOD-65298 | biostudies-arrayexpress
Project description:Individual differences in basal leukocyte gene expression profiles as a function of perceived social isolation (PSI) Gene expression profiling was carried out on peripheral blood mononuclear cell RNA samples collected from 100 adults in Year 10 of the Chicago Health Aging and Social Relations Study. Participants were assessed for PSI using the UCLA Loneliness Scale, and were also classified as chronically high in PSI (1/0) based on UCLA Loneliness Scale scores during study years 1-5. A 1-year lagged value of PSI is also included, and analyses additionally controlled for age at study entry, sex, race/ethnicity (dummy codes for Black and Hispanic), marital status, (log) household income, Body Mass Index (BMI), smoking history, heavy alcohol consumption, depressive symptoms (CESD), perceived stress (PSS), social support (SocSupp), circulating monocyte percentages, and RNA indicators of major leukocyte subsets (CD3E, CD3D, CD4, CD8A, CD19, FCGR3A/CD16, NCAM1/CD56, CD14).
2015-11-23 | E-GEOD-65403 | biostudies-arrayexpress
Project description:Individual differences in basal leukocyte gene expression profiles as a function of perceived social isolation (PSI) Gene expression profiling was carried out on peripheral blood mononuclear cell RNA samples collected from 98 adults in Year 5 of the Chicago Health Aging and Social Relations Study. Participants were assessed for PSI using the UCLA Loneliness Scale, and were also classified as chronically high in PSI (1/0) based on UCLA Loneliness Scale scores during study years 1-5. A 1-year lagged value of PSI is also included, and analyses additionally controlled for age at study entry, sex, race/ethnicity (dummy codes for Black and Hispanic), marital status, (log) household income, Body Mass Index (BMI), smoking history, heavy alcohol consumption, depressive symptoms (CESD), perceived stress (PSS), social support (SocSupp), circulating monocyte percentages, and RNA indicators of major leukocyte subsets (CD3E, CD3D, CD4, CD8A, CD19, FCGR3A/CD16, NCAM1/CD56, CD14).
2015-11-23 | E-GEOD-65213 | biostudies-arrayexpress
Project description:Individual differences in basal leukocyte gene expression profiles as a function of perceived social isolation (PSI) Gene expression profiling was carried out on peripheral blood mononuclear cell RNA samples collected from 92 adults in Year 9 of the Chicago Health Aging and Social Relations Study. Participants were assessed for PSI using the UCLA Loneliness Scale, and were also classified as chronically high in PSI (1/0) based on UCLA Loneliness Scale scores during study years 1-5. A 1-year lagged value of PSI is also included, and analyses additionally controlled for age at study entry, sex, race/ethnicity (dummy codes for Black and Hispanic), marital status, (log) household income, Body Mass Index (BMI), smoking history, heavy alcohol consumption, depressive symptoms (CESD), perceived stress (PSS), social support (SocSupp), circulating monocyte percentages, and RNA indicators of major leukocyte subsets (CD3E, CD3D, CD4, CD8A, CD19, FCGR3A/CD16, NCAM1/CD56, CD14).
2015-11-23 | E-GEOD-65341 | biostudies-arrayexpress