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BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos

ABSTRACT: BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos

PROVIDER: PRJNA967293 | ENA |

REPOSITORIES: ENA

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BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [ATAC-Seq]

Project description:To investigate the effect of BRG1 overexpression in donor cells on the efficiency of porcine somatic cell nuclear transplantation. ATAC-seq results revealed that more open chromatin peaks at SMARCA4、NANOG、SOX2、MAP2K6、HIF1A sites were observed in the experimental group.

2023-05-10 | GSE231684 | GEO

BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [RNA-Seq]

Project description:To investigate the effect of BRG1 overexpression in donor cells on the efficiency of porcine somatic cell nuclear transplantation. In total, 2358 differentially expressed genes were identified by the RNA-seq, with 1336 up- and 1022 down-regulated genes in the experimental group. The up-regulated genes were mainly enriched in PI3K/AKT signaling pathway and WNT signaling pathway, and the down-regulated genes were mainly enriched in disease development.

2023-05-10 | GSE231682 | GEO

BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [ATAC-Seq]

Project description:BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [ATAC-Seq]

| PRJNA967309 | ENA

BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [RNA-Seq]

Project description:BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [RNA-Seq]

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BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-05-10 | GSE231685 | GEO

Chromatin architecture reorganization in somatic cell nuclear transfer embryos

Project description:Chromatin architecture reorganization in somatic cell nuclear transfer embryos

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Histone Demethylase-Assisted Somatic Cell Nuclear Transfer Facilitates Derivation of Human Pluripotent Stem Cells

Project description:The extremely low efficiency of human embryonic stem cell (hESC) derivation using somatic cell nuclear transfer (SCNT) limits potential application. Blastocyst formation from human SCNT embryos occurs at a low rate and with only some oocyte donors. We previously showed in mice that reduction of histone H3 lysine 9 trimethylation (H3K9me3) through ectopic expression of the H3K9me3 demethylase Kdm4d greatly improves SCNT embryo development. Here we show that overexpression of a related H3K9me3 demethylase KDM4A improves human SCNT, and that, as in mice, H3K9me3 in the human somatic cell genome is an SCNT reprogramming barrier. Overexpression of KDM4A significantly improves the blastocyst formation rate in human SCNT embryos by facilitating transcriptional reprogramming, allowing derivation of NTESCs from all oocyte donors tested using adult AMD patient somatic nuclei donors. This conserved mechanistic insight has potential applications for improving SCNT in a variety of contexts, including regenerative medicine. Here we perform RNA-seq based transcriptome profiling in human Donor (fibroblast cells), in vitro fertilized embryos at 8-cell stages (IVF_8Cell), somatic cell nuclear transfer embryos at 8-cell stages (SCNT_8Cell), SCNT assisted by KDM4A 8-cell embryos (SCNT_KDM4A_8Cell). Besides, we also perform RNA-seq in Control human ES cells (CTR_hES) and SCNT assisted by KDM4A derived human ES cells (NTK) with duplicates.Â

2016-03-01 | E-GEOD-73362 | biostudies-arrayexpress

Effect of DJ-1 on early development in porcine nuclear transfer embryos

Project description:We aimed to identify a reprogramming factor in mammalian oocytes. DJ-1 is one candidate gene of the factor. Inhibition of DJ-1 function in nuclear transfer embryos affected developmental abilities. The downstream effect of this DJ-1 inhibition was examined using microarrays. Nuclear transfer (NT) embryos (23-26 embryos per each sample) were collected at 28 h after nuclear transfer when many of the embryos had just reached the 2-cell stage. Total RNA was extracted and hybridized on the Affymetrix GeneChip Porcine Genome Array. Global transcripts were compared among NT embryos injected with anti-DJ1 antibody (αDJ1-NT_1), NT embryos injected with IgG (IgG-NT_1), non-injected NT embryos (NT_1) and donor cells (DonorCell_1). Biologically different samples of αDJ1-NT (αDJ1-NT_2) and IgG-NT (IgG-NT_2) were prepared and the microarray analysis was repeated. In both trials, NT embryos injected with IgG (IgG-NT_1 or IgG-NT_2) were used as controls.

2011-04-07 | E-GEOD-18104 | biostudies-arrayexpress

Genome-wide comparison of EpiSC lines derived from fertilized (FT) and somatic cell nuclear transfer (NT) embryos

Project description:Genome wide comparison of gene expression between EpiSC lines derived from fertilized (FT) embryos and somatic cell nuclear transfer (NT) embryos. EpiSC lines were derived from fertilized and somatic cell nuclear transfer embryos and cultured until 15 to 20 passages. RNA was then extracted in order to compare transcriptomic profiles.

2010-02-05 | GSE17402 | GEO

Transcriptional reprogramming of somatic cells after nuclear transfer to mouse 4-cell embryos

Project description:We have developed a nuclear transfer (NT) system in which somatic nuclei are transplanted into mouse embryos arrested at the 4-cell stage. The transplanted somatic nuclei show swelling and epigenetic reprogramming towards 4-cell-like nuclei. To assess genome-wide transcriptional reprogramming of the injected nuclei, the newly transcribed genes in NT embryos were examined by RNA-seq analyses. As a control, NT was also performed using mouse embryos at the 2-cell stage.

2021-11-03 | GSE162345 | GEO

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