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BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos

ABSTRACT: BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos

PROVIDER: PRJNA967293 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24441907_1.fastq.gz	Fastqsanger.gz
	SRR24441907_2.fastq.gz	Fastqsanger.gz
	SRR24441908_1.fastq.gz	Fastqsanger.gz
	SRR24441908_2.fastq.gz	Fastqsanger.gz
	SRR24441909_1.fastq.gz	Fastqsanger.gz

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BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [ATAC-Seq]

Project description:To investigate the effect of BRG1 overexpression in donor cells on the efficiency of porcine somatic cell nuclear transplantation. ATAC-seq results revealed that more open chromatin peaks at SMARCA4、NANOG、SOX2、MAP2K6、HIF1A sites were observed in the experimental group.

2023-05-10 | GSE231684 | GEO

BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [RNA-Seq]

Project description:To investigate the effect of BRG1 overexpression in donor cells on the efficiency of porcine somatic cell nuclear transplantation. In total, 2358 differentially expressed genes were identified by the RNA-seq, with 1336 up- and 1022 down-regulated genes in the experimental group. The up-regulated genes were mainly enriched in PI3K/AKT signaling pathway and WNT signaling pathway, and the down-regulated genes were mainly enriched in disease development.

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BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [RNA-Seq]

Project description:BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [RNA-Seq]

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BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [ATAC-Seq]

Project description:BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos [ATAC-Seq]

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BRG1 facilitates the development of porcine somatic cell nuclear transfer embryos

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-05-10 | GSE231685 | GEO

Chromatin architecture reorganization in somatic cell nuclear transfer embryos

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Histone Demethylase-Assisted Somatic Cell Nuclear Transfer Facilitates Derivation of Human Pluripotent Stem Cells

Project description:The extremely low efficiency of human embryonic stem cell (hESC) derivation using somatic cell nuclear transfer (SCNT) limits potential application. Blastocyst formation from human SCNT embryos occurs at a low rate and with only some oocyte donors. We previously showed in mice that reduction of histone H3 lysine 9 trimethylation (H3K9me3) through ectopic expression of the H3K9me3 demethylase Kdm4d greatly improves SCNT embryo development. Here we show that overexpression of a related H3K9me3 demethylase KDM4A improves human SCNT, and that, as in mice, H3K9me3 in the human somatic cell genome is an SCNT reprogramming barrier. Overexpression of KDM4A significantly improves the blastocyst formation rate in human SCNT embryos by facilitating transcriptional reprogramming, allowing derivation of NTESCs from all oocyte donors tested using adult AMD patient somatic nuclei donors. This conserved mechanistic insight has potential applications for improving SCNT in a variety of contexts, including regenerative medicine. Here we perform RNA-seq based transcriptome profiling in human Donor (fibroblast cells), in vitro fertilized embryos at 8-cell stages (IVF_8Cell), somatic cell nuclear transfer embryos at 8-cell stages (SCNT_8Cell), SCNT assisted by KDM4A 8-cell embryos (SCNT_KDM4A_8Cell). Besides, we also perform RNA-seq in Control human ES cells (CTR_hES) and SCNT assisted by KDM4A derived human ES cells (NTK) with duplicates.Â

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