Project description:Field-collected and/or laboratory-cultured biomass of the tropical marine benthic filamentous cyanobacterium Moorena bouillonii. Originating from collection sites around Guam, Saipan (Commonwealth of the Northern Mariana Islands), Palmyra Atoll, Papua New Guinea, American Samoa, Kavaratti (Lakshadweep, India), the Paracel Islands (Xisha, China), the Solomon Islands, and the Red Sea (via Egypt). Crude extracts generated with 2:1 dichloromethane and methanol, concentrated and resuspended in acetonitrile, desalted across C18 SPE with acetonitrile. Samples were resuspended in LC-MS grade methanol containing 2uM sulfamethazine as internal standard. LC-MS/MS was performed in an UltiMate 3000 UPLC system (Thermo Scientific) using a Kinetex 1.7 um C18 reversed phase UHPLC column (50 X 2.1 mm) and Maxis Impact Q-TOF mass spectrometer (Bruker Daltonics) equipped with ESI source. Data was acquired in positive ion mode.
Project description:This SuperSeries is composed of the following subset Series: GSE38560: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (RNA-seq) GSE38561: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (ChIP-seq) GSE38562: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (genomic SEQ) GSE38563: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (MNase-seq) GSE38564: CpG islands and GC content dictate nucleosome depletion in a transcription independent manner at mammalian promoters (5) Refer to individual Series