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Differential gene expression in Gaddβ KO mice treated with diethylnitrosamine and phenobarbital

ABSTRACT: Differential gene expression in Gaddβ KO mice treated with diethylnitrosamine and phenobarbital

PROVIDER: PRJNA967695 | ENA |

REPOSITORIES: ENA

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Differential gene expression in Gaddβ KO mice treated with diethylnitrosamine and phenobarbital

Project description:To find Gadd45β dependent gene regulation in mouse hepatocellular carcinoma induced by diethylnitorsamine + phenobarbital

2023-09-25 | GSE231793 | GEO

Transcriptomic profiling of C57BL/APC-KO mice after 9 months treatment with Phenobarbital

Project description:In rodent liver, a single injection of N-nitrosodiethylamine (DEN) followed by chronic treatment with the antiepileptic drug phenobarbital (PB) promotes the outgrowth of hepatocellular tumors with activating mutations in Ctnnb1, encoding the transcription factor β-catenin. We now studied long-term effects of PB treatment in livers of transgenic mice with hepatocyte-specific knockout (KO) of Apc, a negative regulator of β-catenin signaling. The number of Apc KO hepatocytes present in the liver decreased with age, indicative of a selective disadvantage of Apc KO cells in the absence of PB. Following liver tumor promotion by PB in Apc KO mice for 9 months, tumor burden was quantified and histological appearance, gene expression profiles, and activity of oncogenic signaling pathways of the tumors were analyzed. In Apc KO mice fed with PB, we observed an increased hepatic tumor volume fraction and tumor multiplicity, as compared to non-promoted animals. Tumors in the PB-treated Apc KO group were mostly eosinophilic hepatocellular adenoma with activated β-catenin, due to the deletion of Apc. These tumors exhibited striking phenotypic similarities to DEN-induced Ctnnb1-mutated tumors, regarding histological appearance and expression of marker proteins and mRNAs. A particular sub-population of tumors, Apc KO-driven basophilic hepatocellular carcinomas, exclusively appeared in the non-PB-treated group but was absent from PB-promoted livers. In conclusion, phenobarbital promotes the outgrowth of Apc-deficient, β-catenin-activated hepatocellular adenoma while simultaneously inhibiting the formation of a certain population of Apc-driven hepatocellular carcinoma.

2018-05-17 | GSE68969 | GEO

Sf9 induction with phenobarbital

Project description:Effect of phenobarbital on Sf9 cell cultures genes expression. RNA from phenobarbital treated Sf9 cell cultures were compared to control treated (DMSO) Sf9 cell

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Differential Gene Expression in Adgrl3 KO Mice

Project description:Differential Gene Expression in Adgrl3 KO Mice

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Changes in histone modifications H3K4me2, H3k27me3 and H3K36me3 due to phenobarbital in mice

Project description:29-32 days old male mice where either treated with Phenobarbital or untreated

2012-11-10 | GSE41399 | GEO

Effect of Phenobarbital on Rat Primary Hepatocytes.

Project description:This study provides an evaluation of changes in gene expression associated with phenobarbital treatment of rat hepatocytes in vitro. Primary rat hepatocytes were treated for 24 and 48 hours with two doses (300 uM and 3 mM) of phenobarbital and water vehicle control. Five replicates of each treatment were performed. Cells were then extracted and RNA processed for microarray analysis.

2012-08-28 | GSE40339 | GEO

Changes in histone modifications H3K4me2, H3k27me3 and H3K36me3 due to phenobarbital in mice

Project description:29-32 days old male mice where either treated with Phenobarbital or untreated Replicated control/treated study with IP and Input per group and antibody

2012-11-10 | E-GEOD-41399 | biostudies-arrayexpress

Trancriptomic profiling of hepatocytes and mesenchymal cells of mice treated with phenobarbital for 2 weeks

Project description:Conventional notion regards the action of non-genotoxic carcinogens (NGC) an autonomous process largely confined to parenchymal cells. Here we aim to elucidate the role of the hepatic mesenchyme for the action of a prototypical NGC, phenobarbital (PB), an anti-epileptic drug.

2017-07-12 | GSE68111 | GEO

Differential expression of GIF KO vs WT mice

Project description:Differential expression of GIF KO vs WT mice in Liver and Bone with special scope in IGF signaling pathway. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

2013-06-11 | E-ERAD-163 | biostudies-arrayexpress

Microarray experiment to identify gene expression changes in response to Phenobarbital treatment and CncC overexpression in Drosophila.

Project description:Transcriptional profiling of mature adult w1118 males treated with phenobarbital compared to controls and heat treated HS-CncC flies compared with heat treated w1118 flies.

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