Project description:Four new polyhydroxysteroidal glycosides-culcinosides A-D (1, 2, 4, and 7)-along with three known compounds-echinasteroside C (3), linckoside F (5), and linckoside L3 (6)-were isolated from the ethanol extract of starfish Culcita novaeguineae collected from the Xisha Islands of the South China Sea. The structures of new compounds were elucidated through extensive spectroscopic studies and chemical evidence, especially two-dimensional (2D) NMR techniques. The cytotoxicity of the new compounds against human glioblastoma cell lines U87, U251, and SHG44 were evaluated.
Project description:Analysis of U251 glioblastoma multiforme (GBM) cells treated with a new culcita novaeguineae asterosaponion, CN-3. A new asterosaponin was isolated from culcita novaeguineae, an abundant marine resource in the south China sea. The asterosaponin induced significant growth inhibition with a 50% inhibitory concentration at 48 h of 2.013 μg/mL in U251MG cells. 1.8μg/mL of the asterosaponin reduced U251 MG cells viability from 100 % to 42.5% (24 h), 37.4% (48 h) and 52.1% (72 h). In this study, a microarray analysis was performed using RNA prepared from U251MG GBM cells treated with the asterosaponion. These data revealed that 661 genes had significant differential expressions.
