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Nostoc punctiforme

ABSTRACT: Benefits of Nostoc microbiome on the host cyanobacterium

PROVIDER: PRJNA970545 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24556966_1.fastq.gz	Fastqsanger.gz
	SRR24556966_2.fastq.gz	Fastqsanger.gz
	SRR24556967_1.fastq.gz	Fastqsanger.gz
	SRR24556967_2.fastq.gz	Fastqsanger.gz
	SRR24693400_1.fastq.gz	Fastqsanger.gz

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Comparative transcriptomics of wild-type (Nostoc punctiforme ATCC 29133) and hmpD-deletion strains (UCD 543) of Nostoc punctiforme ATCC 29133

Project description:Change in gene expression for a wild-type (Nostoc punctiforme ATCC 29133) and hmpD-deletion strain (UCD 543) of Nostoc punctiforme ATCC 29133 over the time course of hormogonium development This study is further descirbed in Risser, D.D. and Meeks, J.C. 2013. Comparative transcriptomics with a motility deficient mutant leads to identification of a novel polysaccharide secretion system in Nostoc punctiforme. Molecular Microbiology

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Comparative transcriptomics of wild-type (Nostoc punctiforme ATCC 29133) and hmpD-deletion strains (UCD 543) of Nostoc punctiforme ATCC 29133

2013-01-01 | E-GEOD-42859 | biostudies-arrayexpress

Transcriptomic response of Nostoc sp. MG11 under desiccation stress

Project description:To identify the mechanisms of the adaptation to terrestrial ecosystems, an RNA-seq based transcriptome analysis was conducted on a desiccation resistant cyanobacterium, Nostoc sp. MG11.

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Early events of the symbiotic interaction between Oryza sativa and Nostoc punctiforme revealed by a quantitative proteomic approach.

Project description:Plant-cyanobacteria symbiosis is considered one of the pivotal events in the history of life. In this symbiosis, the cyanobacterium provides to the plant fixed nitrogen compounds and plant hormones and, in return, the plant provides to the cyanobacterium fixed carbon. Despite the large knowledge in the physiology and ecology of plant-cyanobacteria symbioses, little is known about the molecular mechanisms involved in the crosstalk between partners. It has been shown recently that Nostoc punctiforme is able to stablish an endophytic symbiosis with Oryza sativa. This finding opens a door to explore this symbiotic interaction as a sustainable alternative to nitrogen fertilization of paddy fields. However, molecular mechanisms behind Oryza-Nostoc endosymbiosis are still not clarified. To gain further insights, an LC-MS/MS based label-free quantitative technique was used to evaluate the differential proteomics under N. punctiforme treatment vs. control plants at 1 day and 7 days. Differential expression profiling reveals a significant number of proteins to be down-regulated or missing in both partners, while others were more abundant or only expressed when both partners were in contact. In N. punctiforme, the differential protein expression was primarily connected to primary metabolism, signal transduction and perception, transport of substances and photosynthesis. In O. sativa, the differential protein expression was connected to a wide range of biological functions regulating carbon and nitrogen metabolism and response to biotic and abiotic stresses.

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Nostoc punctiforme

Project description:Molecular characterization of genes involved in heterocyst differentiation in Nostoc strain

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Transcriptome analysis of a N. punctiforme sigG mutant

Project description:Nostoc punctiforme is a filamentous cyanobacterium capable if differentiating normal vegetative cells into nitrogen fixing heterocysts and spore-like akinetes. Here the effects of a sigma factor (sigG) mutation on the transcriptome was analyzed under 1) normal vegetative growth, and 2) following akinete induction. A zwf strain grown with fructose differentiates into akinetes following transfer to darkness. A DNA microarray was used to compare gene expression from the zwf strain to a zwf sigG double mutant two days following akinte induction by darkness.

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Nostoc punctiforme transcriptome comparison of a SigmaB2 mutant and SigmaB2 overexpression strain

Project description:Sigma factor B2 is one of 13 sigma factors in this cyanobacterium. To determin the SigB2 regulon, transcriptional differences between a sigB2 mutant and overexpression strain were determined in this study.

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