Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq Analysis of Prostate Cancer Cell Under Erastin Exposure

ABSTRACT: RNA-seq Analysis of Prostate Cancer Cell Under Erastin Exposure

PROVIDER: PRJNA970681 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24477009_1.fastq.gz	Fastqsanger.gz
	SRR24477009_2.fastq.gz	Fastqsanger.gz
	SRR24477010_1.fastq.gz	Fastqsanger.gz
	SRR24477010_2.fastq.gz	Fastqsanger.gz
	SRR24477011_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 24

Similar Datasets

RNA-seq Analysis of Prostate Cancer Cell Under Erastin Exposure

Project description:In order to investigate which genes with different expression in prostate cancer(PCa) cells(LNCaP and PC3) after erastin exposure, and identify the essential ferroptosis related genes (FRGs) associated with ferroptosis in PCa cell. 5.0µM erastin was added in cell medium, and we performed gene expression profiling analysis using data obtained from RNA-seq of 12 different cells.

2023-09-12 | GSE232034 | GEO

Identification of ferroptosis related genes and pathways in prostate cancer cells under erastin exposure.

Project description:BackgroundFew studies are focusing on the mechanism of erastin acts on prostate cancer (PCa) cells, and essential ferroptosis-related genes (FRGs) that can be PCa therapeutic targets are rarely known.MethodsIn this study, in vitro assays were performed and RNA-sequencing was used to measure the expression of differentially expressed genes (DEGs) in erastin-induced PCa cells. A series of bioinformatic analyses were applied to analyze the pathways and DEGs.ResultsErastin inhibited the expression of SLC7A11 and cell survivability in LNCaP and PC3 cells. After treatment with erastin, the concentrations of malondialdehyde (MDA) and Fe2+ significantly increased, whereas the glutathione (GSH) and the oxidized glutathione (GSSG) significantly decreased in both cells. A total of 295 overlapping DEGs were identified under erastin exposure and significantly enriched in several pathways, including DNA replication and cell cycle. The percentage of LNCaP and PC3 cells in G1 phase was markedly increased in response to erastin treatment. For four hub FRGs, TMEFF2 was higher in PCa tissue and the expression levels of NRXN3, CLU, and UNC5B were lower in PCa tissue. The expression levels of SLC7A11 and cell survivability were inhibited after the knockdown of TMEFF2 in androgen-dependent cell lines (LNCaP and VCaP) but not in androgen-independent cell lines (PC3 and C4-2). The concentration of Fe2+ only significantly increased in TMEFF2 downregulated LNCaP and VCaP cells.ConclusionTMEFF2 might be likely to develop into a potential ferroptosis target in PCa and this study extends our understanding of the molecular mechanism involved in erastin-affected PCa cells.

| S-EPMC10996193 | biostudies-literature

Differentially expressed genes in pancreatic cancer cell with erastin and vitamin C treatment

Project description:We use RNA Sequencing to analyze the potential molecular mechanisms in human pancreatic cancer cell line BxPC3 cells after erastin and vitamin C treatment for 48h.

2021-12-24 | GSE192362 | GEO

RNA-seq of human pancreatic cancer cell line PANC-1 treated with RSL-3, N6F11, Fin56 and Erastin against untreated controls

Project description:Ferroptotic cell death is dependent on unrestricted lipid peroxidation rather than activation of apoptotic effector caspases. A large number of ferroptosis activators have been reported recently. We used gene sequencing to analyze the effects of four ferroptosis activators (RSL3, N6F11, Fin56 and Erastin) on global gene expression in human PDAC1 cell line.

2023-10-18 | E-MTAB-12241 | biostudies-arrayexpress

RNA transcriptome sequencing analysis of SNU-668 Erastin-resistant cells transfected with shRNA-SOX13 or control shRNA

Project description:a RNA transcriptome sequencing analysis was performed in SNU-668 erastin-resistant cells that were transfected with shRNA-SOX13 or control shRNA

2022-08-18 | GSE211072 | GEO

Microarray analysis of long noncoding RNAs in exosomes from SNU-668 Erastin-resistant cells and parental SNU-668 cells

Project description:a microarray analysis of long noncoding RNAs was performed in exosomes from SNU-668 Erastin-resistant cells and parental SNU-668 cells

2022-09-02 | GSE212303 | GEO

Phosphoproteomic analysis of Hemin- and Erastin-induced neuronal death

Project description:Analysis of the differences in phosphorylation in two ferroptosis subtypes (Hemin vs classical erastin-induced ferroptosis) in neurons, and sensitivity of these changes in phosphorylation levels to treatment with ferroptosis suppressor and MEK inhibitor U0126.

2022-01-28 | PXD029318 | Pride

Maize microbiome under nanosilver exposure

Project description:Metatranscriptome analysis of maize rhizosphere microbiome under nanosilver exposure

| PRJEB22046 | ENA

Per- and Polyfluoroalkyl Substance Exposure Combined with High-Fat Diet Supports Prostate Cancer Progression

Project description:Per- and polyfluoroalkyl substances (PFAS) are synthetic chemicals utilized in various industrial settings, and include products such as flame retardants, artificial film-forming foams, cosmetics, non-stick cookware among others. Epidemiological studies suggest a link between increased blood PFAS levels and prostate cancer incidence, but the mechanism through which PFAS impact cancer development is unclear. To investigate the link between PFAS and prostate cancer, we evaluated the impact of metabolic alterations resulting from a high-fat diet combined with PFAS exposure on prostate tumor progression. Our studies are the first in the field to provide new and clinically relevant insights regarding novel metabolic and epigenetic states and support future development of effective preventative and therapeutic strategies for PFAS-induced prostate cancers. Our findings enhance understanding of how PFAS synergize with high-fat diets to contribute to prostate cancer development and establish an important basis to mitigate PFAS exposure.

2021-12-01 | GSE185183 | GEO

Human BAT/WAT under Cold Exposure/Thermoneutrality

Project description:Human BAT/WAT under Cold Exposure/Thermoneutrality

| PRJEB11666 | ENA