Project description:Transcriptional gene silencing (TGS) in Arabidopsis is regulated by two distinct pathways, DNA methylation-dependent and –independent. ddm1 and mom1 mutants are defective in the DNA-dependent and –independent pathway, respectively. We used microarrays to detail the global changes in gene expression in the TGS mutants Keywords: TGS mutants
Project description:We grafted Transcriptional Gene Silencing (TGS)-inducing wild type Arabidopsis and a mutant that is compromised in 24 nucleotide (nt) small RNA (sRNA) production onto a wild type reporter line. We observed that 21-24 nt sRNAs were transmitted across a graft union yet only the 24 nt sRNAs directed RNA-dependent DNA methylation (RdDM) and TGS of a transgene promoter in meristematic cells.
Project description:To identify genes of the guard cell transkriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. Ost1-2 and slac1-3 mutants were compared to their wildtype.
