Project description:To determine whether acLDL uptake was associated with specfifc patterns of gene expression we treated vasculogenic mimicry-competent 4T1-T mammary carcinoma cells with Alexa-488 labelled acetylated low density lipoproteins (acLDL) and sorted the cells based on high or low uptake and subjected them to RNA-Seq
Project description:Molecular comparison between control 4T1 cells with MMP3-low 4T1 cells RNA extracted from biological triplicates of each of the above mentioned cell populations were subjected to microarray analysis
Project description:Comparative analysis of the transcriptome of 4T1 cells stably transduced with a lentiviral vector expressing a siRNA against murine SPARC (4T1-C18) with 4T1 control cells stably transduced with a lentiviral vector expressing a scramble sequence (4T1-SCR). Two-condition experiment, 4T1-C18 vs. 4T1-SCR cells. Biological replicates: 4 SPARC knock down, 4 control, independently grown in vitro and harvested. One replicate per array. Microarrays were hybridized in three different days.
Project description:The extracellular matrix has been shown to control breast epithelial cell morphogenesis, proliferation and signalling. Here we compared changes in gene expression between 4T1 murine mammary carcinoma cells grown in a low or high density collagen matrix