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Komagataella phaffii strain:CBS7435

ABSTRACT: Komagataella phaffii Raw sequence reads + assembly + bigWigs

PROVIDER: PRJNA971544 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR25445902_1.fastq.gz	Fastqsanger.gz
	SRR25445902_2.fastq.gz	Fastqsanger.gz
	SRR25445903_1.fastq.gz	Fastqsanger.gz
	SRR25445903_2.fastq.gz	Fastqsanger.gz
	SRR25445904_1.fastq.gz	Fastqsanger.gz

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Crosslinking Mapping of yeast Komagataella phaffii TFIID complex

Project description:Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. We used CXMS to study the organization and structure of the purified TFIID complex from Komagataella phaffii. Together with Cryo-EM, We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a HF domain. Interaction with promoter DNA highlights two non-selective binding sites consistent with a DNA scanning mode.

2018-10-08 | PXD011092 | Pride

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