Project description:Examination of the difference in mRNA expression profile between mid-old fibroblasts co-cultured with mid-old fibroblasts and mid-old fibroblasts co-cultured with young fibroblasts
Project description:Investigation of the rejuvenation effect to mid old cell, we co-cultured mid old cell with young cell or mid old cells. After 30days we harvest cells and extrect RNA then anlaysis the gene expression.
Project description:MiRNA expression profile was assessed in cardiac fibroblasts isolated from young (2 months old) and aged (16 months old) mice, by next generation sequencing of small RNA. Bioinformatic analysis of next-generation sequencing output demonstrated 88 differentially expressed miRNAs in aged mice - derived cardiac fibroblasts versus young mice-derived cells, of which two thirds were downregulated and one third were upregulated. The majority of downregulated miRNAs were mapped to chromosome 12, as part of Meg3-Mirg locus, while the remaining were spread on all chromosomes. Almost 90% of miRNA genes within Meg3-Mirg locus were expressed over a wide range in cardiac fibroblasts and had a decreasing trend in the aged group, the majority being significantly downregulated. This study provides evidence that aging is associated to significant alterations in miRNA expression profile in cardiac fibroblasts, a predominant feature being the downregulation of miRNAs within Meg3-Mirg locus.
Project description:This study comprises DNA methylation profiles of 9 directly converted human fibroblasts from young, adult and old donors into oligodendrocytes (dc-hiOL).
Project description:Mass spectrometry was performed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific) interfaced with an UltiMate 3000 Binary RSLCnano System (Dionex). Proteome Discoverer v.1.4 (Thermo Scientific) with SEQUEST HT search engines was used for the spectra-preprocessing and HCD MS2 spectra were used for peptide identification and quantitation based on TMT reporter ions. TMT isobaric comparison of old versus young haematopoietic stem and progenitor cells. Young 1 and Young 2 are samples 126 and 128 of dataset UTH_1. Old 1 and Old 2 are samples 129 and 130 of UTH_1. Young 3 is sample 131 and Old 3 is sample 130 of dataset UTH_4.
Project description:To identify the gene expression change during pituitary aging, we did the RNA-seq of pituitary from 2-3 months young mice and 18-24 months old mice. The mRNA level of different genes were analyzed.