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Murine ESC-derived basal cell (iBC) 5th generation secondary transplantation

ABSTRACT: Murine ESC-derived basal cell (iBC) 5th generation secondary transplantation

PROVIDER: PRJNA972700 | ENA |

REPOSITORIES: ENA

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Murine ESC-derived basal cell (iBC) 5th generation secondary transplantation

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murine ESC-derived basal cell (iBC) transplantation (40&56 days post-transplantation)

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in vitro murine iBC culture

Project description:The goal of this experiment is to study mouse pluripotent stem cell-derived airway basal cell (iBC) culture at a single cell resolution.

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Transcriptomic analysis of A3250 inflammatory breast cancer (IBC) cells, SUM149 IBC and MDA-MB-231 non-IBC cells

Project description:Purpose: Transcriptome profiling (RNA-seq) of a novel human Inflammatory Breast Cancer cell line A3250 in comparison to SUM149 and MDA-MB-231 Inflammatory Breast Cancer (IBC) is the most aggressive form of breast cancer with distinct clinical and histopathological features, but understanding of the unique aspects of IBC biology lags far behind that of other breast cancers. We describe a novel triple-negative IBC cell line, A3250, that recapitulates key features of human IBC in a mouse xenograft model.The purpose of this study was to compare differences in gene expression between A3250 IBC, MDA-MB-231 non-IBC and SUM149 IBC that does not present with typical clinical sympotms of IBC in a mouse model, with the goal of identifying unique molecular features for this unique type of breast cancer Results: RNA-Seq analysis identified expression profile characteristic for the novel A3250 IBC cell line, compared to SUM149 IBC and MDA-MB-231 non-IBC.

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