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ABSTRACT: Haplotype-resolved T2T genome assembly of Ziziphus jujuba 'Junzao' and Ziziphus jujuba var. spinosa

PROVIDER: PRJNA974227 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	SRR26446806_1.fastq.gz	Fastqsanger.gz
	SRR26446807_1.fastq.gz	Fastqsanger.gz
	SRR26446808_1.fastq.gz	Fastqsanger.gz
	SRR26446809_1.fastq.gz	Fastqsanger.gz

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Project description:Constructing high-quality haplotype-resolved genome assemblies has substantially improved the ability to detect and characterize genetic variants. A targeted approach providing readily access to the rich information from haplotype-resolved genome assemblies will be appealing to groups of basic researchers and medical scientists focused on specific genomic regions. Here, using the 4.5 megabase, notoriously difficult-to-assemble major histocompatibility complex (MHC) region as an example, we demonstrated an approach to construct haplotype-resolved assembly of the targeted genomic region with the CRISPR-based enrichment. Compared to the results from haplotype-resolved genome assembly, our targeted approach achieved comparable completeness and accuracy with reduced computing complexity, sequencing cost, as well as the amount of starting materials. Moreover, using the targeted assembled personal MHC haplotypes as the reference both improves the quantification accuracy for sequencing data and enables allele-specific functional genomics analyses of the MHC region. Given its highly efficient use of resources, our approach can greatly facilitate population genetic studies of targeted regions, and may pave a new way to elucidate the molecular mechanisms in disease etiology.

2022-11-15 | GSE192501 | GEO

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