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Chemical induction of hepatocyte revitalization in injured human hepatocytes [RNA-seq 3]

ABSTRACT: Chemical induction of hepatocyte revitalization in injured human hepatocytes [RNA-seq 3]

PROVIDER: PRJNA974869 | ENA |

REPOSITORIES: ENA

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Chemical induction of hepatocyte revitalization in mouse hepatocytes [RNA-seq 2]

Project description:We examined how each chemical contributed to hepatocyte revitalization by removing each component of the 5C induction cocktail individually. We then compared deach chemical contributed to hepatocyte revitalization by removing each component of the 5C induction cocktail individually

2023-11-21 | GSE233082 | GEO

Chemical induction of hepatocyte revitalization in injured human hepatocytes [RNA-seq 3]

Project description:3C cocktail could revitalize human hepatocytes displaying apoptotic and EMT phenotypes induced by TGF-β, and promote hepatocyte proliferation and maintenance

2023-11-21 | GSE233083 | GEO

Chemical induction of hepatocyte revitalization in mouse hepatocytes [RNA-seq 2]

Project description:Chemical induction of hepatocyte revitalization in mouse hepatocytes [RNA-seq 2]

| PRJNA974870 | ENA

Chemical induction of hepatocyte revitalization in mouse hepatocytes [RNA-seq 1]

Project description:Chemical induction of hepatocyte revitalization in mouse hepatocytes [RNA-seq 1]

| PRJNA974868 | ENA

Chemical induction of hepatocyte revitalization in mouse hepatocytes [RNA-seq 1]

Project description:We then compared whole transcriptome bulk RNA-seq data of dHeps isolated from CCl4-injured mice and treated with either 5C, 3C, or DMSO. The expression of hepatocyte-enriched TFs and hepatocyte functional genes was upregulated in both the 5C and 3C groups, while mesenchymal markers (Acta2, Mmp3), inflammatory-related genes (Cxcl10, Ecm1, Ccl6, and Cd68), and oxidative stress-related genes (Ncf2 and Ncf1), were down-regulated compared with their expression in the DMSO control group

2023-11-21 | GSE233081 | GEO

Chemical induction of liver progenitor cells from mature hepatocytes

Project description:Chemical induction of liver progenitor cells from mature hepatocytes

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Heterogeneity of hepatocyte dynamics restores liver architecture after chemical, physical or viral damage

Project description:The liver has a remarkable capacity for regeneration after injury. Midlobular hepatocytes have been proposed as the most plastic hepatic cell type, providing definitive evidence that zone 2 of the liver lobule acts as a reservoir for hepatocyte proliferation during homeostasis and regeneration. However, the implication of other mechanisms beyond hyperplasia that contribute to liver repair have been little explored and the collaboration of another hepatocyte subpopulation has differed among previous studies depending on the model of liver injury used. Thus, re-examination of dynamics of hepatocytes during regeneration is critical to get a better understanding of underlaying mechanisms for potential cell therapy and treatment of liver diseases. Here, using a mouse model of hepatocyte- and non-hepatocyte-specific multicolor lineage tracing, we demonstrate that hepatocytes located in the midlobular region also undergo hypertrophy besides cell division in response to chemical, physical, and viral insults. Our study shows for first time that this subpopulation also combats liver impairment after infection with coronavirus. Furthermore, we demonstrate that pericentral hepatocyte subpopulation also expands in number and size during the repair process in collaboration with midlobular hepatocytes and Galectin-9-CD44 pathway may be critical for driving these processes. Interestingly, we identified transdifferentiation and cell fusion processes during liver regeneration after severe injury that may be key to recover hepatic function.

2024-01-12 | GSE250568 | GEO

Gene expression profile of hepatocytes and macrophages at single cell level in normal and DDC-injured 1 week liver [scRNA-seq]

Project description:By employing single-cell transcriptional profiling, we uncovered that mature hepatocytes re-activate reprogramming/progenitor-related genes (RRG) and dedifferentiate to liver progenitor-like cells (LPLC). Because we found macrophages regulated hepatocyte dedifferentiation, We also dissected the transcriptional profiling of hepatocytes in single-cell level during DDC injury after macrophage-depletion via clondronate liposomes. Besides, we uncovered the heterogeneity of liver macrophages in normal and DDC-injured liver via scRNA-seq as well.

2022-09-04 | GSE212692 | GEO

Miz1 Limits Tumor-promoting Function of Hepatocyte NF-κB independently of Its Transcriptional Activity in Chemical Hepatocarcinogenesis

Project description:NF-κB has a crucial tumor-suppression role in chemical hepatocarcinogenesis (HCC) by preventing hepatocyte apoptosis-induced compensatory proliferation. However, NF-κB is typically activated in chemical HCC animal models and in ~40% HCC patients, in which its role in tumor progression is largely not known. Here we report that transcription factor Miz1 limits tumor-promoting function of NF-κB independently of its transcriptional activity in chemical HCC. In a murine model, hepatocyte-specific deletion of Miz1 exacerbates HCC progression. Miz1 loss results in a unique sub-group of hepatocytes with upregulated NF-κB activity and pro-inflammatory cytokine production, skewing infiltrating macrophages toward M1-like pro-inflammatory phenotype. Mechanistically, Miz1 sequestrates and prevents IKK-phosphorylation of Metadherin (MTDH), thereby inhibiting NF-κB nuclear translocation and transcription activity. In HCC patient specimens, Miz1 expression is inversely correlated with phosphorylation of RelA and MTDH, and poor prognosis. Thus, Miz1 preventing hepatocytes from promoting infiltrating macrophage M1-like phenotype and inflammation in chemical HCC progression.

2021-07-13 | GSE142868 | GEO

Human hepatocyte metaplasia in injured humanized mouse livers

Project description:Human hepatocyte metaplasia in injured humanized mouse livers

| PRJNA253143 | ENA