Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Determining genomic localization of SIX1 in EWS-502 cells with SIX1 depletion

ABSTRACT: Determining genomic localization of SIX1 in EWS-502 cells with SIX1 depletion

PROVIDER: PRJNA975062 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24684585_1.fastq.gz	Fastqsanger.gz
	SRR24684585_2.fastq.gz	Fastqsanger.gz
	SRR24684586_1.fastq.gz	Fastqsanger.gz
	SRR24684586_2.fastq.gz	Fastqsanger.gz
	SRR24684587_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 20

Similar Datasets

Determining genomic localization of SIX1 in EWS-502 cells with SIX1 depletion

Project description:Investigating genomic binding of SIX1 in EWS-502 cells transduced with shSCR or one of two shSIX1 shRNAs to deplete SIX1.

2023-05-22 | GSE233118 | GEO

Effect of shRNA-mediated knockdown of SIX1 in EWS-502 cells in vitro

Project description:To investigate gene expression changes associated with SIX1 in the EWS-502 cell line

2023-05-22 | GSE215354 | GEO

Determining genomic localization of SIX1 in A673 cells with SIX1 depletion

Project description:Determining genomic localization of SIX1 in A673 cells with SIX1 depletion

| PRJNA889827 | ENA

Effect of shRNA-mediated knockdown of SIX1 in EWS-502 cells in vitro

Project description:Effect of shRNA-mediated knockdown of SIX1 in EWS-502 cells in vitro

| PRJNA889835 | ENA

Determining genomic localization of SIX1 in A673 cells with SIX1 depletion

Project description:Investigating genomic binding of SIX1 in A673 cells transduced with shSCR or one of two shSIX1 shRNAs to deplete SIX1.

2023-05-22 | GSE215350 | GEO

Synechococcus sp. CCFWC 502 isolate:CCFWC 502

Project description:Synechococcus sp. CCFWC 502 isolate:CCFWC 502 Genome sequencing and assembly

| PRJNA880272 | ENA

A functional liaison between E2F and the aberrant ETS oncogene EWS/FLI1 in Ewing's sarcoma

Project description:Translocations of ETS transcription factors are driver mutations in diverse cancers. We investigated the genomic network of the ETS fusion EWS/FLI1 in Ewing's sarcoma (ESFT) as a model of ETS-driven tumorigenesis. ChIP-Seq and transcriptional analysis identified E2F3 as a principle co-factor of EWSFLI1 defining functionally distinct gene sets. While EWS/FLI1 binding independent of E2F3 predominantly associated with repressed differentiation genes, significant co-localization with E2F3 was discovered at proximal promoters of activated growth-related genes. Thus, EWS/FLI1 promotes oncogenesis by simultaneously perturbing differentiation state and augmenting the expression of genes co-regulated by E2F3. Integration of additional E2F3 and ERG localization data from prostate cancer containing TMPRSS2/ERG verified that the ETS-E2F module is also found in prostate cancer and may be of general relevance to ETS driven cancers. Timecourse with 6 timepoints of a doxicyclin inducible EWS-FLI1 knockdown in the A673 Ewing's Sarcoma celline

2013-07-01 | E-GEOD-27524 | biostudies-arrayexpress

Ectomesenchymal Six1 controls mandibular skeleton formation [CUT&Tag]

Project description:Craniofacial development requires intricate cooperation of multiple transcription factors and signaling pathways. Six1 is a critical transcription factor regulating craniofacial development. However, the exact function of Six1 during craniofacial development remains elusive. In this study, we investigated the function of Six1 in mandible development using a Six1 knockout mouse model (Six1-/-) and a cranial neural crest-specific, Six1 conditional knockout mouse model (Six1f/f; Wnt1-Cre). The Six1-/- mice exhibited multiple craniofacial deformities, including severe microsomia, high-arched palate, and uvula deformity. Importantly, the Six1f/f; Wnt1-Cre mice recapitulate the microsomia phenotype of Six1-/- mice, thus demonstrating that the expression of Six1 in ectomesenchyme is critical for mandible development. We further showed that the knockout of Six1 led to abnormal expression of osteogenic genes within the mandible. Moreover, the knockdown of Six1 in C3H10 T1/2 cells reduced their osteogenic capacity in vitro. Using RNA-seq, we showed that both the loss of Six1 in the E18.5 mandible and the Six1 knockdown in C3H10 T1/2 led to the dysregulation of genes involved in embryonal skeletal development. In particular, we showed that Six1 binds to the promoter of Bmp4, Fat4, Fgf18, and Fgfr2, and promotes their transcription. Collectively, our results suggest that Six1 plays a critical role in the regulation of mandibular skeleton formation during mouse embryogenesis.

2023-03-10 | GSE216760 | GEO

Ectomesenchymal Six1 controls mandibular skeleton formation [RNA-seq]

2023-03-10 | GSE216759 | GEO

Differentiation resistant HPV16-immortalized keratinocytes: Six1 transfected vs. Control.

Project description:The overexpression of Six1, a member of the Six family of homeodomain transcription factors, has been found in various human cancers, and is associated with tumor progression and metastasis. We previously determined that the expression of Six1 mRNA increased during in vitro progression of human papillomavirus type 16 (HPV16)-immortalized human keratinocytes (HKc/HPV16) toward a differentiation-resistant (HKc/DR) phenotype. However, if Six1 promotes HPV16-mediated transformation or not remains unknown. HKc/DR were transfected with a Six1 or control vector and RNA isolated from these cells were used in an Agilent two-color gene expression profiling experiment. The goal was to determine the effects of Six1 on global gene expression. Two-condition experiment, Six1 vs. Control HKc/DR cells. Biological replicates: 4 Six1-transfected replicates and 4 control-transfected replicates.

2013-12-24 | E-GEOD-53589 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data