Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Low-input Hi-C library construction

ABSTRACT: Low-input Hi-C library construction

PROVIDER: PRJNA975160 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24709560_1.fastq.gz	Fastqsanger.gz
	SRR24709560_2.fastq.gz	Fastqsanger.gz
	SRR24709561_1.fastq.gz	Fastqsanger.gz
	SRR24709561_2.fastq.gz	Fastqsanger.gz
	SRR24709562_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 14

Similar Datasets

Low-input Hi-C library construction

Project description:To evaluate the robustness of CtG, we constructed Hi-C libraries of varying quantities of cell inputs utilizing a low-input Hi-C technique.

2023-07-19 | GSE233166 | GEO

Low input proteomics on FABP7 hi/low mammary luminal progenitor cells

Project description:Low input proteomics on FABP7 hi/low mammary luminal progenitor cells isolated from human patient samples

2023-12-11 | MSV000093639 | MassIVE

Fast Low-Input Efficient Hi-C

Project description:Fast Low-Input Efficient Hi-C

| PRJNA1157835 | ENA

Fast Low-Input Efficient Hi-C

Project description:We developed FLIE (Fast Low-Input Efficient) Hi-C, a highly optimized and simplified version of Hi-C. Our method shortens the duration of Hi-C from five days to two days and decreases key reagent amounts 5-20 fold, while maintaining experimental controls and data quality. Importantly, the method also reduces the required input material by 100-1000 fold, as we demonstrate by generating detailed high complexity interaction maps from low-input sorted mouse frozen neuron nuclei and from 5,000-10,000 human Hap1 cells.

2024-09-06 | GSE276553 | GEO

Low input LC ISC

Project description:R&D project to develop low input library construction methods.

| EGAS00001001856 | EGA

Low input LC WGS

Project description:R&D project to develop low input library construction methods.

| EGAS00001001855 | EGA

Microfluidic platform for next-generation sequencing library preparation with low-input samples

Project description:Advances in Next Generation Sequencing (NGS) have made available a wealth of information that had previously been inaccessible to researchers and clinicians. NGS has been applied to understand genomic, transcriptomic, and epigenomic changes and gained traction as a significant tool capable of accelerating diagnosis, prognosis, and biomarker discovery. However, these NGS assays have yet to be practical methods for patient stratification or diagnosis because of the gap between the tiny quantities of biomaterials provided by a clinical sample and the large DNA input required by most of these assays. Current library preparation methodologies typically require large input amounts of DNA and a long and complicated manual process. Here we present a microfluidic reactor system for NGS library preparation, capable of reducing the number of pipetting steps significantly, automating much of the process, while supporting extremely low DNA input requirement (10 pg per library). This largely automated technology will allow for low-input preparations of 8 libraries simultaneously while reducing batch to batch variation and operator hands-on time.

2020-01-02 | GSE132786 | GEO

Systematic assessment of commercially available low-input miRNA library preparation kits

Project description:Systematic assessment of commercially available low-input miRNA library preparation kits

| PRJNA552326 | ENA

Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.

Project description:Rapid, low-input, low-bias construction of shotgun fragment libraries by high-density in vitro transposition.

| PRJNA274948 | ENA

Microfluidic platform for next-generation sequencing library preparation with low-input samples

Project description:Microfluidic platform for next-generation sequencing library preparation with low-input samples

| PRJNA548967 | ENA