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Effect of nano-hydroxyapatite and phosphate on thorium toxicity – Arabidopsis transcriptomic study

ABSTRACT: Effect of nano-hydroxyapatite and phosphate on thorium toxicity – Arabidopsis transcriptomic study

PROVIDER: PRJNA976333 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24740300_1.fastq.gz	Fastqsanger.gz
	SRR24740300_2.fastq.gz	Fastqsanger.gz
	SRR24740301_1.fastq.gz	Fastqsanger.gz
	SRR24740301_2.fastq.gz	Fastqsanger.gz
	SRR24740302_1.fastq.gz	Fastqsanger.gz

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Effect of nano-hydroxyapatite and phosphate on thorium toxicity – Arabidopsis transcriptomic study

Project description:Thorium (Th) is a ubiquitous element but elevated concentrations caused by anthropogenic or natural activities pose health and environmental risks. Nowadays, Th is obtained mainly as by-product during the mining and extraction rare earth element. However, due to its abundance in nature, which is higher than the abundance of uranium, Th is considered as a potential fuel for the reactors. Therefore, in this study, we focused on the effect of thorium on Arabidopsis plants at the transcriptomic level. Since Th and phosphorus form insoluble phosphates, we focused on the effect of Th in the presence/absence of phosphate. Furthermore, we investigated the effect of hydroxyapatite nanoparticles, which are considered as material for in situ remediation, on Th toxicity. Not surprisingly, the highest number of DEGs was recorded in the combination of P absence and Th presence followed by the presence of Th in the standard hydroponic medium, the absence of P in the medium, the presence of nano-hydroxyapatite, and the presence of Th together with hydroxyapatite. Besides a high number of genes involved in stress response, Th also induced the transcription of genes involved in response to P starvation, indicating that Th decreased the availability of P. P deficiency caused by Th contributed to the phytotoxic effect of Th. Nano-hydroxyapatite significantly reduced Th toxicity. Nano-hydroxyapatite also served as a source of inorganic P for the plants, but caused deficiency of essential metals indicated by up-regulation of genes involved in their acquisition.

2023-06-24 | GSE233480 | GEO

Novel insights into pulmonary phosphate homeostasis and osteoclastogenesis emerge from the study of pulmonary alveolar microlithiasis.

Project description:Pulmonary alveolar microlithiasis (PAM) is an autosomal recessive lung disease caused by a deficiency in the pulmonary epithelial Npt2b sodium-phosphate co-transporter that results in accumulation of phosphate and formation of hydroxyapatite microliths in the alveolar space. The single cell transcriptomic analysis of a PAM lung explant showing a robust osteoclast gene signature in alveolar monocytes and the finding that calcium phosphate microliths contain a rich protein and lipid matrix that includes bone resorbing osteoclast enzymes suggested a role for osteoclast-like cells in the defense against microliths.

2022-09-26 | GSE199329 | GEO

Nano genome altas (NGA) of body wide tissue specific responses

Project description:Purpose: In order to detect the unpredictable risks of the cumulative nanoparticles infiltrated everywhere in the whole body after systematic administration Methods: By using RNA-seq, we systematically studied the transcriptomic response of multi-organs from mice and tissue specific (stem) cells to the hydroxyapatite (HA) nano-delivery system. Results: We show that HA could differentially influence gene expression, immune response, cell proliferation, stress, apoptosis, metabolic, ion transport activity, cell signaling and epigenetic activity across tissues and tissue specific (stem) cells.

2019-03-29 | GSE125742 | GEO

Inorganic phosphate solubilization by rhizosphere bacterium Paenibacillus sonchi genomovar Riograndensis SBR5: gene expression and physiological functions

Project description:Due to the importance of phosphorus (P) in agriculture, crop inoculation with phosphate solubilizing bacteria (PSB) is a relevant subject of study. Paenibacillus sonchi genomovar Riograndensis SBR5 is a promising candidate for crop inoculation as it can fix nitrogen and excrete ammonium in a remarkably high rate. However, its trait of phosphate solubilization (PS) has not yet been studied in detail. Here, differential gene expression and functional analyses were performed in order to characterize PS in this bacterium. SBR5 was cultivated with two distinct P sources: NaH2PO4 as soluble phosphate source (SPi) and hydroxyapatite as insoluble phosphate source (IPi). Total RNA of SBR5 cultivated in those two conditions was isolated and sequenced and bacterial growth and product formation were monitored. In the IPi medium, the expression of 68 genes was upregulated, while 100 genes were downregulated. Among those, genes involved in carbon metabolism, including those coding for subunits of 2-oxoglutarate dehydrogenase were identified. Quantitation of organic acids showed that the production of tricarboxylic acid cycle-derived organic acids was reduced in IPi condition, while acetate and gluconate were overproduced. Increased concentrations of proline, trehalose, and glycine betaine revealed active osmoprotection during growth in IPi. The cultivation with hydroxyapatite also caused the reduction in the motility of SBR5 cells as a response to Pi depletion in the beginning of its growth. SBR5 was able to solubilize hydroxyapatite, which suggests that this organism is a promising PSB. Our findings are the initial step in the elucidation of the PS process in P. sonchi SBR5 and will be a valuable groundwork for further studies of this organism as a plant growth promoting rhizobacterium.

2021-01-13 | GSE154303 | GEO

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2014-07-17 | GSE59470 | GEO

Tricresyl Phosphate (CAS No. 1330-78-5): 5-Day Rat Gavage Toxicogenomic Study

Project description:Tricresyl Phosphate (TCP) is an organophosphate flame retardant currently on the market and used as a replacement for phased-out polybrominated diphenyl ethers. Toxicity information on this class of chemicals is inadequate. Herein, the NTP use short-term, in vivo transcriptomic studies to inform potential points of departure for TCP.

2022-07-01 | GSE123433 | GEO

Triphenyl Phosphate (CAS No. 115-86-6): 5-Day Rat Gavage Toxicogenomic Study

Project description:Triphenyl phosphate (TPHP) is an organophosphate flame retardant currently on the market and used as a replacement for phased-out polybrominated diphenyl ethers. Toxicity information on this class of chemicals is inadequate. Herein, the NTP use short-term, in vivo transcriptomic studies to inform potential points of departure for TPHP.

2018-10-23 | GSE121134 | GEO

Isopropylated Phenol Phosphate (CAS No. 68937-41-7): 5-Day Rat Gavage Toxicogenomic Study

Project description:Isopropylated Phenol Phosphate (IPP) is an organophosphate flame retardant currently on the market and used as a replacement for phased-out polybrominated diphenyl ethers. Toxicity information on this class of chemicals is inadequate. Herein, the NTP use short-term, in vivo transcriptomic studies to inform potential points of departure for IPP.

2022-07-01 | GSE123432 | GEO

Isodecyl Diphenyl Phosphate (CAS No. 29761-21-5): 5-Day Rat Gavage Toxicogenomic Study

Project description:Isodecyl Diphenyl Phosphate (IDDP) is an organophosphate flame retardant currently on the market and used as a replacement for phased-out polybrominated diphenyl ethers. Toxicity information on this class of chemicals is inadequate. Herein, the NTP use short-term, in vivo transcriptomic studies to inform potential points of departure for IDDP.

2022-07-01 | GSE123431 | GEO

Insights into pulmonary phosphate homeostasis and osteoclastogenesis emerge from the study of pulmonary alveolar microlithiasis

Project description:Pulmonary alveolar microlithiasis is an autosomal recessive lung disease caused by a deficiency in the pulmonary epithelial Npt2b sodium-phosphate co-transporter that results in accumulation of phosphate and formation of hydroxyapatite microliths in the alveolar space. The single cell transcriptomic analysis of a pulmonary alveolar microlithiasis lung explant showing a robust osteoclast gene signature in alveolar monocytes and the finding that calcium phosphate microliths contain a rich protein and lipid matrix that includes bone resorbing osteoclast enzymes and other proteins suggested a role for osteoclast-like cells in the host response to microliths. While investigating the mechanisms of microlith clearance, we found that Npt2b modulates pulmonary phosphate homeostasis through effects on alternative phosphate transporter activity and alveolar osteoprotegerin, and that microliths induce osteoclast formation and activation in a receptor activator of nuclear factor kappa B (NF-kB) ligand and dietary phosphate dependent manner. This work reveals that Npt2b and pulmonary osteoclast like cells play key roles in pulmonary homeostasis and suggest potential new therapeutic targets for the treatment of lung disease.

2023-02-16 | MSV000091302 | MassIVE

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