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ScRNA-seq of mouse Plasmodium-specific TCR-transgenic CD4+ T cells prior to and after re-infection.

ABSTRACT: scRNA-seq of mouse Plasmodium-specific TCR-transgenic CD4+ T cells prior to and after re-infection.

PROVIDER: PRJNA977623 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24764160.fastq.gz	Fastqsanger.gz
	SRR24764161.fastq.gz	Fastqsanger.gz
	SRR24764162.fastq.gz	Fastqsanger.gz
	SRR24764163.fastq.gz	Fastqsanger.gz

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scRNA-seq of mouse Plasmodium-specific TCR-transgenic CD4+ T cells prior to and after re-infection.

Project description:Naturally-acquired immunity to blood-stage malaria is associated with several effector CD4 + T subsets including germinal centre (GC) Tfh, Th1 and Tr1 cells. Children in malaria-endemic regions can experience repeated Plasmodium infections over short periods of time; yet the effect of reinfection on multiple co-existing effector and memory subsets remains unclear. Here, we tracked antigen-experienced TCR-transgenic CD4+ T cells during Plasmodium re-infection in mice using scRNA-seq.

2024-05-02 | GSE233703 | GEO

scRNA-seq + TCR-seq of mouse polyclonal CD4+ T cells prior to and after re-infection.

Project description:Naturally-acquired immunity to blood-stage malaria is associated with several effector CD4 + T subsets including germinal centre (GC) Tfh, Th1 and Tr1 cells. Children in malaria-endemic regions can experience repeated Plasmodium infections over short periods of time; yet the effect of reinfection on multiple co-existing effector and memory subsets remains unclear. Here, we tracked antigen-experienced polyclonal CD4+ T cells during Plasmodium re-infection in mice using scRNA-seq/TCR-seq.

2024-05-02 | GSE233713 | GEO

scRNA-seq + TCR-seq of mouse polyclonal CD4+ T cells prior to and after re-infection.

Project description:scRNA-seq + TCR-seq of mouse polyclonal CD4+ T cells prior to and after re-infection.

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scRNA-seq of CD4+ T cells prior to and after re-infection.

Project description:scRNA-seq of CD4+ T cells prior to and after re-infection.

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Id proteins suppress E2A-driven iNKT cell development prior to TCR selection [RNA-seq]

Project description:Id proteins have been shown to promote the differentiation of conventional αβ and γδT cells, and to suppress the expansion of invariant Natural Killer T (iNKT) cells and innate-like γδNKT within their respective cell lineages. However, it remains to be determined whether Id proteins regulate lineage specification in developing T cells that give rise to these distinct cell fates. Here we report that in the absence of Id2 and Id3 proteins, E2A prematurely activates genes critical for the iNKT cell lineage prior to TCR expression. Lack of Id proteins also promotes a biased TCR rearrangement in favor of iNKT cell fate prior to selection at the CD4+CD8+ double positive (DP) stage. Enhanced iNKT development in Id3-deficient mice lacking γδNKT cells suggests that Id3 regulates the lineage competition between these populations. RNA-Seq analysis establishes E2A as the transcriptional regulator of both iNKT and γδNKT development. In the absence of pre-TCR signaling, Id2/Id3 deletion gives rise to a large population of iNKT cells and a unique innate-like DP population, despite the block in conventional αβ T cell development. The transcriptional profile of these unique DP cells reflects enrichment of innate-like signature genes, including PLZF (Zbtb16) and Granzyme A (Gzma). Results from these genetic models and genome-wide analyses suggest that Id proteins suppress E2A-driven innate-like T cell programs prior to TCR selection to enforce predominance of conventional T cells.

2018-01-23 | GSE89848 | GEO

Id proteins suppress E2A-driven iNKT cell development prior to TCR selection [ChIP-seq]

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Id proteins suppress E2A-driven iNKT cell development prior to TCR selection [RNA-seq_new]

2018-01-23 | GSE100601 | GEO

Id proteins suppress E2A-driven iNKT cell development prior to TCR selection

Project description:Id proteins suppress E2A-driven iNKT cell development prior to TCR selection

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LC-MS analysis of Murine eIF4E-BP1/2 Double Knockout naive and TCR stimulated CD4+ T cells

Project description:This project focuses on examining the differences in the global CD4+ T cell proteome between cells lacking the negative regulators of eIF4E( eIf4E-Binding Proteins 1/2) and WT naive CD4+ T cells both prior and following TCR stimulation.

2023-05-20 | PXD039635 | Pride

Proteome of CD4 T cells activated through the TCR in the presence of IL2 and IL12 - Part 1

Project description:To activate primary T cells, lymph nodes were removed and disaggregated. Cells were cultured in RPMI 1640 containing L-glutamine, 10% FBS, 50 μM β-mercaptoethanol and penicillin/streptomycin. Cells were stimulated with 1 μg/ml of the CD3 monoclonal antibody (2C11) and 2 μg/ml anti-CD28 (37.51) in the presence of cytokines IL12 (10 ng/ml) and 20 ng/ml IL2 (20 ng/ml). Cells were stimulated for 24 hours. Live TCR activated CD4+ cells were sorted for CD4+ and CD45.1+ expression and DAP1 exclusion prior to collection for proteomics processing. Mice were “wild-type” (ie non-TCR transgenic) from OT-2 CD45.1 expressing C57bl6 background maintained in-house line.

2019-03-29 | PXD012052 | Pride

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