Project description:AGO-PAR-CLIP was employed to identify microRNA binding sites in BCBL-1, a Kaposi's sarcoma-associated herpesvirus (KSHV) infected B-cell line and DG75, a KSHV negative B-cell line as a control. By using our novel computational method (PARma) and differential analysis of PAR-CLIP data, highly accurate target sites of KSHV microRNAs can be defined.
Project description:AGO-PAR-CLIP was employed to identify microRNA binding sites in BCBL-1, a Kaposi's sarcoma-associated herpesvirus (KSHV) infected B-cell line and DG75, a KSHV negative B-cell line as a control. By using our novel computational method (PARma) and differential analysis of PAR-CLIP data, highly accurate target sites of KSHV microRNAs can be defined. Examination of microRNA target sites in two different cell lines using replicate PAR-CLIP experiments
Project description:<p>The Genetics of Congenital Anomalies of the Kidney and Urinary Tract (CAKUT) contributes data from unrelated individuals who were recruited at the Columbia University Medical Center, NY; the Polish Registry of Congenital Malformations, Poland; the Universities of Parma, Bari and Brescia and the Gaslini Insitute, in Italy; the University of Skopje, Macedonia; and VUMC of Amsterdam, in The Netherlands. These individuals consented to share their data for scientific discovery. The purpose of the study is to investigate the genetic architecture of CAKUT. Genomic DNA samples were genotyped on the Illumina Multi-Ethnic Global Array (MEGA), designed by the MEGA Consortium.</p>