Project description:A study of the miRNA profile CD4 T cells compared to T reg cells; of activated and naive T cells; and the miRNA profile conferred by enforced expression of Foxp3. Keywords: cell type comparison Fifteen arrays broken down into three broader experiments as referenced by series GSE6003 - a comparison of miRNA profile of natural T reg cells with conventional CD4+CD25- T cells; GSE6006 - a comparison of miRNA profile of activated T cells to naive T cells; and GSE6007 - comparison of miRNA profile of Foxp3-transduced activated T cells with control vector-transduced activated T cells.
Project description:Activated T cells were transduced with either Foxp3-IRES-GFP or IRES-GFP control vector. Low molecular weight RNA from cells was extracted 72h or 96h later and hybridised to microarrays containing oligonucleotide probes corresponding to known miRNA sequences. Keywords: cell type comparison
Project description:Activated T cells were transduced with either Foxp3-IRES-GFP or IRES-GFP control vector. Low molecular weight RNA from cells was extracted 72h or 96h later and hybridised to microarrays containing oligonucleotide probes corresponding to known miRNA sequences. Keywords: cell type comparison Four arrays representing two timepoints dyeswaps of each.
Project description:A study of the miRNA profile CD4 T cells compared to T reg cells; of activated and naive T cells; and the miRNA profile conferred by enforced expression of Foxp3. Keywords: cell type comparison
Project description:E47 represses Foxp3 transcription, albeit indirectly through the activation of unknown negative regulatory of Foxp3 transcription. To identify target genes of E47 in regulatory T cells, we compared gene expression profiles of Treg cells overexpressing E47 compared to control-vector transduced cells.
Project description:Analysis of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells. Results indicate regulatory T cell (Treg) ontogenesis requires two independent processes, expression of the transcription factor Foxp3 and establishment of Treg epigenetic programs induced by T cell receptor (TCR) stimulation. GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis (Affymetrix, mouse genome 430 2.0 array). To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector. Two replicates each.
Project description:Foxp3-mediated gene expression program in resting vs. activated CD4+ T cells The microarray part of this work consists of 12 Affymetrix assays corresponding to 3 biological replicates for each of 4 conditions for CD4+CD25- cells. Cells were either transduced with an empty vector or a vector encoding FOXP3, and in each case cells were either stimulated or not by anti-CD3/anti-CD28.
