Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution

ABSTRACT: GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution

PROVIDER: PRJNA978429 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24791673.fastq.gz	Fastqsanger.gz
	SRR24791674.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution

Project description:We developed a quantitative method called GLORI to investigate m6A methylation in the mammalian transcriptome at single-base resolution.

2023-06-18 | GSE233875 | GEO

Absolute quantification of single-base m6A methylation in the mammalian transcriptome

Project description:To investigate the m6A methylation in the mammalian transcriptome, we developed a quantitative method, names GLORI, that could detect m6A stoichiometry at single-base resolution. We then performed GLORI on cells with different treatment, such as stress, knockdown and inhibitor. We next analysis m6A methyloms of different celllines and cells under different treatment to investigate the fuctional role of m6A.

2022-08-06 | GSE210563 | GEO

Absolute quantification of single-base m6A methylation in the mammalian transcriptome

Project description:Absolute quantification of single-base m6A methylation in the mammalian transcriptome

| PRJNA866163 | ENA

Absolute quantification of miRNAs

Project description:Absolute quantification of miRNAs

| PRJNA116765 | ENA

Absolute quantification of mammalian microRNAs

Project description:Absolute quantification of mammalian microRNAs

| PRJNA1140118 | ENA

Genome-wide absolute quantification of chromatin looping

Project description:Genome-wide absolute quantification of chromatin looping

| PRJNA1209796 | ENA

Absolute quantification of miRNAs

Project description:MicroRNAs (miRNAs) have been shown to play an important role in many different cellular, developmental, and physiological processes. Accordingly, numerous methods have been established to identify and quantify miRNAs. The shortness of miRNA sequence results in a high dynamic range of melting temperatures and, moreover, impedes a proper selection of detection probes or optimized PCR primers. While miRNA microarrays allow for massive parallel and accurate relative measurement of all known miRNAs, they have so far been less useful as an assay for absolute quantification. Here, we present a microarray based approach for global and absolute quantification of miRNAs. The method relies on an equimolar pool of about 1000 synthetic miRNAs of known concentration which is used as an universal reference and labeled and hybridized in a dual colour approach on the same array as the sample of interest. Each single miRNA is quantified with respect to the universal reference outbalancing bias related to sequence, labeling, hybridization or signal detection method. We demonstrate the accuracy of the method by various spike in experiments. Further, we quantified miRNA copy numbers in liver samples and CD34(+)CD133(-) hematopoietic stem cells.

2009-10-27 | GSE15835 | GEO

m6A-Label-seq directly reports m6A methylome at base resolution

Project description:Here we report a metabolic labeling method to map mRNA N6-methyladenosine (m6A) modification transcriptome-wide at base resolution, termed m6A-label-seq. The cells were fed with Se-allyl-L-selenohomocysteine, an analog of methoine, which serves as the precursor of methylation enzyme cofactor, so that cellular RNAs were continuously deposited with N6-allyladenosine (a6A) at supposed m6A sites. We enriched a6A-containing mRNAs and sequenced their a6A sites which are identical to m6A sites, based on iodination-induced misincorporation during reverse transcription.

2020-04-21 | GSE131316 | GEO

m6A-Label-seq directly reports m6A methylome at base resolution

Project description:m6A-Label-seq directly reports m6A methylome at base resolution

| PRJNA543237 | ENA

Absolute quantification of CD34(+)/CD133(-) hematopoietic stem cells

Project description:Absolute quantification of CD34(+)/CD133(-) hematopoietic stem cells

| PRJNA116757 | ENA