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Circ_0000235 Targets MCT4 to Promote Glycolysis and Progression of Bladder Cancer by Sponging miR-330-5p

ABSTRACT: Circ_0000235 Targets MCT4 to Promote Glycolysis and Progression of Bladder Cancer by Sponging miR-330-5p

PROVIDER: PRJNA978602 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24798088_1.fastq.gz	Fastqsanger.gz
	SRR24798088_2.fastq.gz	Fastqsanger.gz
	SRR24798089_1.fastq.gz	Fastqsanger.gz
	SRR24798089_2.fastq.gz	Fastqsanger.gz
	SRR24798090_1.fastq.gz	Fastqsanger.gz

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Circ_0000235 Targets MCT4 to Promote Glycolysis and Progression of Bladder Cancer by Sponging miR-330-5p

Project description:Warburg effect plays a crucial role in bladder cancer (Bca) development. However, the mechanism by which glycolysis is involved in Bca remains poorly understood. CircRNAs commonly play a regulatory role in tumor progression. Our study discovered and identified a novel circRNA, hsa_circ_0000235 (circ235), and investigated its role in the glycolytic process, which further results in the progression of Bca. We applied qRT-PCR to assess its clinicopathological relevance and evaluated its proliferation, migration, and glycolytic capacity. We investigated its mechanism using RNA immunoprecipitation, dual-luciferase reporters, and fluorescence in situ hybridization. The findings demonstrated that circ235 was dramatically increased in Bca tissues and was related to a worse prognosis. In vitro studies revealed that circ235 accelerated the rate of extracellular acidification and promoted glucose uptake and lactate manufacture in Bca cells. Additionally, it strengthened the capacity of Bca cells to proliferate and migrate. Experiments on animals revealed that downregulating circ235 dramatically reduced carcinogenesis and tumor growth. Cir235 activates monocarboxylate transporter 4 (MCT4) by sponging miR-330-5p, which promotes glycolysis and tumor growth. In conclusion, these findings imply that circ235 may be a viable molecular marker and therapeutic site for Bca.

2023-08-09 | GSE233933 | GEO

Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p.

Project description:Warburg effect plays a crucial role in bladder cancer (Bca) development. However, the mechanism by which glycolysis is involved in Bca remains poorly understood. CircRNAs commonly play a regulatory role in tumor progression. Our study discovered and identified a novel circRNA, hsa_circ_0000235 (circ235), and investigated its role in the glycolytic process, which further results in the progression of Bca. We applied qRT-PCR to assess its clinicopathological relevance and evaluated its proliferation, migration, and glycolytic capacity. We investigated its mechanism using RNA immunoprecipitation, dual-luciferase reporters, and fluorescence in situ hybridization. The findings demonstrated that circ235 was dramatically increased in Bca tissues and was related to a worse prognosis. In vitro studies revealed that circ235 accelerated the rate of extracellular acidification and promoted glucose uptake and lactate manufacture in Bca cells. Additionally, it strengthened the proliferative and migratory capacities. Experiments on animals revealed that downregulating circ235 dramatically reduced carcinogenesis and tumor growth. Circ235 activates monocarboxylate transporter 4 (MCT4) by sponging miR-330-5p, which promotes glycolysis and tumor growth. In conclusion, these findings suggest that circ235 may be a viable molecular marker and therapeutic target for Bca.

| S-EPMC10397263 | biostudies-literature

Rpph1 regulated Cdc42 and modulated hippocampal pyramidal neurons by competing endogenous miR-330-5p

Project description:To build up competing endogenous RNA regulatory network based on APP/PS1 transgenic mice model and verify Rpph1, miR-330-5p and Cdc42 interaction

2017-06-10 | GSE87550 | GEO

CircSry regulates spermatogenesis by enhancing γH2AX expression via sponging miR138-5p

Project description:CircSry regulates spermatogenesis by enhancing γH2AX expression via sponging miR138-5p

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Long noncoding RNA Linc00673 which enhances cancer cells progression by sponging miR-515-5p to elevate expression of MARK4 in human breast cancer

Project description:In the present study, Linc00673 displayed high expression in breast cancer tissues and was associated with poor prognosis of breast cancer patients. Importantly, Linc00673 depletion inhibited breast cancer cell proliferation by impairing cell cycle progression and apoptosis. Mechanically, RNA sequencing was performed to investigate the potential crosstalk between Linc00673 and its targets. We found that Linc00673 acted as a competing endogenous RNA by sponging miR-515-5p to regulate MARK4 expression which inhibits the Hippo signaling pathway to promote proliferation of breast cancer.

2019-10-29 | GSE133331 | GEO

Silenced LINC01963 enhanced the chemosensitivity of prostate cancer cells to docetaxel by sponging miR-216b-5p to inhibit TrkB protein level

Project description:Long noncoding RNAs (lncRNAs) affect docetaxel chemosensitivity. However, the biological role and regulatory mechanisms of lncRNAs in docetaxel-resistant prostate cancer remain unclear. Differences in lncRNAs were evaluated by lncRNA sequencing and evaluated using quantitative real-time polymerase chain reaction, and TrkB expression was measured through Western blot analysis. Proliferation was measured using the MTS, while apoptosis and cell cycle were measured using flow cytometry. Additionally, migration and invasion were measured using transwell assays. Forty-eight female BALB/c nude mice were used for subcutaneous tumorigenicity and lung metastasis assays. We found that LINC01963 was overexpressed in the PC3-DR cells. LINC01963 silencing enhanced the chemosensitivity of PC3-DR to docetaxel and inhibited tumorigenicity and lung metastasis, while LINC01963 overexpression enhanced the chemoresistance of PC3 cells to docetaxel. It was found that LINC01963 sponges miR-216b-5p. The miR-216b-5p inhibitor reversed the suppressive effect of sh-LINC01963 on PC3-DR cell proliferation, migration, and invasion. Furthermore, miR-216b-5p can bind to the 3’-UTR of NTRK2 and inhibit TrkB protein levels. TrkB enhances docetaxel resistance in prostate cancer and reverses the effects of LINC01963 silencing and miR-216b-5p overexpression. In conclusion, silencing LINC01963 enhanced the chemosensitivity of PC3-DR to docetaxel by sponging miR-216b-5p to inhibit TrkB protein levels.

2021-12-13 | GSE190648 | GEO

Hsa_circ_0001021 regulates intestinal epithelial barrier function via sponging miR-224-5p in ulcerative colitis

Project description:Hsa_circ_0001021 regulates intestinal epithelial barrier function via sponging miR-224-5p in ulcerative colitis

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Tulasnella sp. 330

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Circular RNA hsa_circ_0000848 promotes trophoblast cell migration and invasion and inhibits cell apoptosis by sponging hsa-miR-6768-5p

Project description:To investigate the expression profiles of circRNAs in the placental tissues from pregnant women with fetal growth restriction (FGR), Arraystar Human circRNA Array V2 analysis was performed. Six samples (three samples each from FGR group and healthy controls group) were used in the microarray screening. 13532 circRNAs were identified in the placental tissues from FGR and normal groups, and only 244 circRNAs were found to be more differentially expressed in the placental tissues of the FGR group than in normal placenta (fold-change > 1.5, p < 0.05). Among them, 100 and 144 circRNAs were up- and down-regulated, respectively. To verify circRNA expression in the FGR placenta, we chose hsa_circ_0007738, hsa_circ_0071271, and hsa_circ_0000848, which have the greatest expression differences between FGR and normal groups, for further analysis. Hsa_circ_0000848 expression level was significantly lower in FGR placental tissues than in the normal placenta tissues, whereas hsa_circ_0007738 and hsa_circ_0071271 expression levels did not change significantly. Further, we found that hsa_circ_0000848 promoted trophoblast cell migration and invasion, and inhibited cell apoptosis via sponging miR-6768-5p.

2020-03-31 | GSE147721 | GEO

lncRNA n384546 promotes thyroid papillary cancer proliferation and migration by targeting miR-145-5p to regulate AKT3

Project description:we document a novel lncRNA, n384546, which may exert its oncogenic property in PTC tumorigenesis by sponging miR-145-5p and then regulating its target AKT3.This study reveals that n384546 is an oncogenic lncRNA in human thyroid cancer.

2019-06-16 | GSE124841 | GEO

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