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Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia

ABSTRACT: Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia

PROVIDER: PRJNA979138 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24820300_1.fastq.gz	Fastqsanger.gz
	SRR24820300_2.fastq.gz	Fastqsanger.gz
	SRR24820301_1.fastq.gz	Fastqsanger.gz
	SRR24820301_2.fastq.gz	Fastqsanger.gz
	SRR24820302_1.fastq.gz	Fastqsanger.gz

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Similar Datasets

Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia [scRNA-Seq]

Project description:Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia [scRNA-Seq]

| PRJNA979139 | ENA

Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia [RNA-Seq]

Project description:Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia [RNA-Seq]

| PRJNA979140 | ENA

Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia [scRNA-Seq]

Project description:β-catenin-dependent canonical Wnt signaling plays a plethora of roles in organ development, tissue homeostasis and cancer. Here we identified an upstream enhancer of Ctnnb1 - ieCtnnb1 - that controls intestinal homeostasis. ieCtnnb1 is specifically active in crypts of small and large intestines. Single-cell sequencing revealed that ieCtnnb1 knockout (ieCtnnb1KO) biased epithelial composition and functions of small intestinal crypts – leaning toward absorptive functions at the expense of secretive roles. Deletion of ieCtnnb1 hampered epithelial turnovers in physiologic and regenerative conditions. In contrast, deletion of ieCtnnb1 prevents occurrence and progression of Wnt/β-catenin driving colorectal cancers. The human ieCTNNB1 specifically drives reporter in intestinal crypts and contains a SNP that is associated with CTNNB1 expression levels in human gastrointestinal epithelia. The enhancer activity of ieCTNNB1 in colorectal cancer tissues is higher than that in adjacent normal tissues and positively correlates with CTNNB1 expression levels. Key trans-factors that bind to ieCTNNB1 and regulate CTNNB1 transcription were identified. Together, these findings revealed an enhancer-dependent mechanism that controls the dosage of Wnt signaling, hence homeostasis of intestinal epithelia.

2024-10-02 | GSE233978 | GEO

Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia [RNA-Seq]

2024-10-02 | GSE233977 | GEO

Wnt signaling dosage controlled by a Ctnnb1 enhancer balances homeostasis and tumorigenesis of intestinal epithelia

Project description:This SuperSeries is composed of the SubSeries listed below.

2024-10-02 | GSE233979 | GEO

Dosage compensation in the mouse balances up regulation and silencing of X-linked genes

Project description:Dosage compensation in mammals involves silencing of one X chromosome in XX females, and requires expression, in cis, of Xist silencing RNA. Using microarray analysis to assay expression of X-linked genes in mouse embryonic stem cells, we show that dosage compensation also involves global up-regulation of expression from the active X in both males and females. Up-regulation is complete (ie. 2-fold) only after 2-3 weeks of differentiation. X-linked gene silencing in female cells occurs on a gene-by-gene basis throughout differentiation and includes a sub-group of genes silenced prior to the onset of differentiation and independently of enhanced Xist expression. Keywords: dosage compensation, gene silencing, Xist RNA, microarray

2007-12-01 | GSE8593 | GEO

Correct dosage of X chromosome transcription is controlled by a nuclear pore component

Project description:Correct dosage of X chromosome transcription is controlled by a nuclear pore component

| PRJNA649320 | ENA

Transcriptomic analysis of gastric cancer cells in response to stable over-expression of circular RNA derived from CTNNB1 gene (circ-CTNNB1)

Project description:Circular RNAs (circRNAs), a subclass of noncoding RNAs characterized by covalently closed continuous loops, play emerging roles in tumorigenesis and aggressiveness. However, the functions and underlying mechanisms of circRNAs in regulating the Wnt/β-catenin signaling and cancer progression still remain elusive. We identify one intronic circRNA derived from CTNNB1 (circ-CTNNB1) as a novel driver of cancer progression. To investigate the mechanisms underlying the oncogenic functions of circ-CTNNB1, we employed the Illumina HiSeq X Ten as a discovery platform to analyze the transcriptome profiling changes of human gastric cancer AGS cells in response to stable over-expression of circ-CTNNB1. The results showed that stable over-expression of circ-CTNNB1 led to altered expression of 2230 human mRNAs, including 1244 up-regulated genes and 986 down-regulated genes. Furthermore, we validated the RNA-seq results by real-time RT-PCR with high identity. Overall, our results provided fundamental information about the transcriptomic changes in response to circ-CTNNB1 over-expression in human cancer cells, and these findings will help us understand the pathogenesis of cancer progression.

2019-09-30 | GSE120496 | GEO

Identification of Wnt target genes associated with malignant phenotypes in CTNNB1-mutated NSCLC

Project description:Microarray analysis comparing the expression profiles of siRNA-mediated CTNNB1-silenced and CTNNB1-retained HCC15 cells harboring a homozygous CTNNB1 S45F mutation revealed that besides several known Wnt genes, LGR6 was significantly downregulated by CTNNB1 knockdown in HCC15 cells.

2022-09-01 | GSE183178 | GEO

Ctnnb1-targeted genes in mouse post-meiotic germ cells

Project description:To determine differential gene expression in Ctnnb1 CKO mice testis compared to CTRL mice testis. The Agilent Mouse Whole Genome 4x44K array (Samples are from pooled total testes RNA from 4 CTRL and 4 Ctnnb1 CKO mice; dye-swap was performed.)

2012-03-20 | E-GEOD-30773 | biostudies-arrayexpress

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