Project description:To investegate how IL6 regulates human monocyte transcriptional profiles, we stimulated primary human monocytes in vitro with IL6 and IL6R.
Project description:In this study 6 cell lines originated from liver, skin and colon tumours as well as 3 non-tumour cell lines from the same organs were profiled with and without stimulation by 4 cytokines: IFNg, hyper-IL6, IL27 and OSM. The main objective was investigation of downstream effects of the stimulation if these cancer cell lines as well as in healthy tissues.
Project description:We investigate the impact of an initial stimulation and a re-stimulation in altering transcriptional responses in both peripheral blood-monocyte derived macrophages and human pluripotent stem cell derived macrophages.
