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The study of nascent RNA transcripts in asynchronous cells and cells released from mitosis at the indicated timepoints between IAA or dmso treatment in the SMARCE1 - AID system

ABSTRACT: The study of nascent RNA transcripts in asynchronous cells and cells released from mitosis at the indicated timepoints between IAA or dmso treatment in the SMARCE1 - AID system

PROVIDER: PRJNA980097 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24827503_1.fastq.gz	Fastqsanger.gz
	SRR24827503_2.fastq.gz	Fastqsanger.gz
	SRR24827504.fastq.gz	Fastqsanger.gz
	SRR24827505.fastq.gz	Fastqsanger.gz
	SRR24827506.fastq.gz	Fastqsanger.gz

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The study of nascent RNA transcripts in asynchronous cells and cells released from mitosis at the indicated timepoints between IAA or dmso treatment in the SMARCE1 - AID system

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The study of nascent RNA transcripts in asynchronous cells and cells released from mitosis at the indicated timepoints between Smarce1- MD (R42A) and Smarce1- MD mESCs.

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Transcription profiling of Smarce1- AID mESCs , cultured the cells with IAA treatment from 0min to 7 days.

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Project description:To evaluate the differential potential affected by SMARCE1 -MD/MD(R42A) , we performed RNA-sequencing (RNA-seq) of Smarce1-MD and control Smarce1-MD (R42A) embrynoic body.

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Project description:To evaluate the effects of mitotic degradation of SMARCE1 upon gene expression, we performed RNA-sequencing (RNA-seq) of cultures of four independent subclones each of Smarce1-MD and control Smarce1-MD (R42A) mESCs. We found that transcription of the core pluripotency regulatory network was not disrupted. In contrast, GO analysis showed that neural differentiation-associated terms were enriched among genes upregulated in Smarce1-MD mESCs. To better understand the difference in neural fates in the neural induction experiments, we performed differential gene expression analysis and gene set enrichment analysis (GSEA) studies. Mitotic degradation of SMARCE1 resulted in higher expression of GABA receptors and hyper-activation of synaptic signaling on neural induction, indicating the aberrant neural cell fate commitment compared to SMARCE1-MD (R42A) cultures. And then we add back BMP4 to partically rescue the phenotype and very small amount of BMP4 will rescue the phenotype.

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