Transcriptomic profile of 3D spheroids developed from Astyanax Liver-derived cells - SFL and CFL.
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ABSTRACT:
PROVIDER: PRJNA980547 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24835119.fastq.gz | Fastqsanger.gz | |||
| SRR24835120.fastq.gz | Fastqsanger.gz | |||
| SRR24835121.fastq.gz | Fastqsanger.gz | |||
| SRR24835122.fastq.gz | Fastqsanger.gz | |||
| SRR24835123.fastq.gz | Fastqsanger.gz |
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Project description:HT-29 and HCT-116 cells were barcoded using the CloneTracker lentiviral barcode library and then dabrafenib and irinotecan resistant derivatives of these cell lines were established, respectively.10 million barcoded HT-29 and HCT-116 cells were seeded equally onto poly-HEMA coated 4xT75 flask (DMSO Control, Replica A, B, C for each drug). After seeding, cells were allowed to form spheroids and barcoded 3D-HT-29 spheroids were treated with dabrafenib at increasing doses starting from IC50/10 dose until IC50/2 dose with monthly doubling of the dosing (16 weeks), and barcoded 3D-HCT-116 cells were treated with irinotecan at increasing doses starting from IC50/4 dose until IC50 dose with weekly doubling of the dosing (4 weeks). Following the end points of treatment for each cell line, DNA was isolated from harvested cell lines and barcode sequencing and whole exome sequencing were carried out.
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