Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:This work investigated the relationship of host size, seasons, and water quality parameters with the prevalence and intensity of Cornudiscoides agarwali on Mystus bleekeri collected from the Dikrong River in Arunachal Pradesh, India from February 2016 to January 2017. A total of 2760 specimens of C. agarwali were recovered from 114 individuals of M. bleekeri. The levels of mean intensity, but not the prevalence, of infection of C. agarwali were positively correlated with fish host size, peaking in the largest size class (45.20 ± 5.69 parasites/fi sh). The prevalence values had a statistically significant seasonal trend, reaching highest (100 %) during the pre-monsoon season, followed by 91.8% during the post-monsoon period and 87.5 % during the monsoon season. The levels of mean intensity of infection were also dependent on the seasons, reaching significantly higher levels during the pre-monsoon season (42.75 ± 4.18 parasites/fi sh). All water quality parameters measured were within the safety value recommended for freshwater aquaculture. Cornudiscoides agarwali maintained its prevalence above 87.5 % throughout the annual cycle, which means it was able to reproduce year-round in a non-polluted river. This could be an indication of monogenoidean community and population dynamics thriving best under optimum water quality parameters. Also, this article draws the attention of parasitologists and ichthyologists to a taxonomic problem of the misidentification of Mystus spp., and therefore, possibly of their parasitic monogenoids.
Project description:Mystus gulio Hamilton, also called long whiskers catfish, is an endemic fish and a common food in some Asian countries. In this study, the complete mitochondrial genome of M. gulio was sequenced using the MinION system (Oxford Nanopore Technologies). The mitochondrial genome is 16,518 bp in length (41.1% [G + C] content) and consists of 13 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. The results of phylogenetic analysis inferred from whole mitochondrial genomes of Mystus and related species in the family Bagridae revealed that M. gulio is closely related to Mystus cavasius.