Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Sj cDNAs

PROVIDER: PRJNA982300 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24914363_1.fastq.gz	Fastqsanger.gz
	SRR24914363_2.fastq.gz	Fastqsanger.gz
	SRR24914364_1.fastq.gz	Fastqsanger.gz
	SRR24914364_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

RACK7 ChIP-seq in pediatric glioblastomas cell lines.

Project description:We identified that RACK7 recognizes the histone H3.3G34R mutaion in vitro. In order to determine the interaction between RACK7 and H3.3G34R mutaion in cells, we used three pediatric glioblastoma (pGBM) cell lines. SJ-HGGx6c(R6) and SJ-HGGx42c(R42) have heterozygous G34R mutation, while SJ-HGGx39c(WT39) has wildtype H3F3A, which encodes H3.3, and we also corrected H3.3G34R in R6 and R42 cells to wildtype H3.3 by CRISPR/Cas9 mediated knock-in. Finally, we performed RACK7 ChIP-seq in these cell lines to explore the function of RACK7 and H3.3G34R mutation.

2020-08-10 | GSE138060 | GEO

Transcriptome analysis of females and males following knockdown serine/threonine protein phosphatase 1 catalytic subunit encoding genes(Sj-pp1c) in Schistosoma japonicum

Project description:Purpose: To obtain the differentially expressed genes based on post-RNAi transcriptional profiling analysis of females and males, which provide a more comprehensive overview about the effects of Sj-pp1c knockdown on schistosoma developmental and reproduction biology at the molecular level. Methods: Schistosoma japonicum females and males were collected from 21 days post infection worm pairs that untreated (Blank,BLA_FE/M) or treated with combined Sj-pp1c dsRNAs (Sj-pp1c KD,PP1_FE/M) cultured for 7 d in vitro. Total mRNA profiles of females and males were generated by deep sequencing, in triplicate, using Illumina HiSeqTM2000. Results: 41-51 million reads (average 44 million) were obtained for each sample after quality filtering, and 63-67% reads were mapped to S. japonicum reference genome(WormBase ParaSite, http://parasite.wormbase.org/ftp.html; version 7.0) and identified 15,314 transcripts by Tophat (version v2.0.12) and Cufflinks (Version 2.1.1.). 2291 and 229 transcripts showed differentially expressed in males and females, respectively,between the untreated and Sj-pp1c dsRNAs treated group, with adjust P value <0.05. GO analysis showed differentially expressed genes are mainly function in extracellular matrix structural constituent, proteolysis and collagen trimer. Conclusions: We performed analysis on transcriptional profiling of females and males following Sj-pp1c-RNAi by RNA-seq, which provided molecular evidence for the pleiotropic roles that Sj-pp1c plays in schistosome biology.

2018-06-24 | GSE108738 | GEO

Genome-wide transcriptomic analysis in pediatric glioblastomas cell lines by RNA-seq.

Project description:We identified that RACK7 recognizes the histone H3.3G34R mutaion in vitro and in vivo. In order to explore the function of RACK7 and H3.3G34R mutaion, we used three pediatric glioblastomas(pGBM) cell lines. SJ-HGGx6c(R6) and SJ-HGGx42c(R42) have heterozygous G34R mutation, while SJ-HGGx39c(WT39) has wildtype H3F3A, which encodes H3.3. We next corrected H3.3G34R in R6 and R42 cells to wildtype H3.3 by CRISPR/Cas9 mediated knock-in, and knocked out RACK7 in R6 and R42 cells by CRISPR/Cas9. Finaly, we performed genome-wide transcriptomic analysis in these cell lines by RNA-seq analysis.

2020-08-10 | GSE138077 | GEO

Mucilaginibacter sp. SJ strain:SJ

Project description:Mucilaginibacter sp. SJ strain:SJ Genome sequencing and assembly

| PRJNA935958 | ENA

Project description:Sj miRNA inhibition

| PRJNA1166905 | ENA

Microcystis aeruginosa Sj strain:Sj

Project description:Microcystis aeruginosa Sj

| PRJDB5694 | ENA

Gene expression profiles of mESCs

Project description:The 24th recoverable satellite of China was designed specifically for experiments on microgravity physics and space life science that are collectively referred to as the SJ-10 programme. We participated in the SJ-10 programme using Rad9-/- mESCs and corresponding wild-type mESCs as study models, comparing the gene expression profiles of these models after 1 day or 5 days of culture in space to those of their counterparts on the ground

2019-08-31 | GSE134090 | GEO

Nitrosopumilus sp. SJ

Project description:Nitrosopumilus sp. SJ genome sequencing

| PRJNA66413 | ENA

Bacillus sp. SJ-10

Project description:Bacillus sp. SJ-10 Genome sequencing

| PRJNA421716 | ENA

Encephalitozoon romaleae SJ-2008

Project description:Encephalitozoon romaleae SJ-2008 Genome sequencing

| PRJNA160949 | ENA