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MNase-Seq analysis of wild-type Candida glabrata strain in RPMI-growth and macrophage-internalized conditions at 2 h and 10 h post-infection.

ABSTRACT: MNase-Seq analysis of wild-type Candida glabrata strain in RPMI-growth and macrophage-internalized conditions at 2 h and 10 h post-infection.

PROVIDER: PRJNA982736 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24895600_1.fastq.gz	Fastqsanger.gz
	SRR24895600_2.fastq.gz	Fastqsanger.gz
	SRR24895593_1.fastq.gz	Fastqsanger.gz
	SRR24895593_2.fastq.gz	Fastqsanger.gz
	SRR24895594_1.fastq.gz	Fastqsanger.gz

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MNase-Seq analysis of wild-type Candida glabrata strain in RPMI-growth and macrophage-internalized conditions at 2 h and 10 h post-infection.

Project description:MNase-sequencing analysis led to identification of 56,637 to 71,823 nucleosomes under RPMI-growth or macrophage-internalized conditions. Mapping of dynamic nucleosomes revealed that 12 to 24% of total nucleosomes were altered in their position, occupancy or fuzziness. Notably, while the nucleosome position shift was the most common dynamic event, the nucleosome fuzziness was the least observed change. Promoter regions were found to be nucleosome-depleted.

2024-04-16 | GSE234671 | GEO

RNA-Seq analysis of Candida glabrata wild-type and Cgsnf2Δ strains in RPMI-grown or macrophage-internalized conditions at 2 h and 10 h post-infection.

Project description:The goal of the current study was to identify differentially-expressed genes upon CgSNF2 deletion, as well as, upon macrophage internalization in C. glabrata wild-type (wt) and Cgsnf2Δ strains. For this study, RNA samples were collected from RPMI-grown and macrophage-internalized cells of C. glabrata wild-type and Cgsnf2Δ strains at 2 h and 10 h post-infection. Comparative transcriptome analysis shows the differential regulation of 1419 genes in C. glabrata wild-type cells upon macrophage internalization, whereas the CgSNF2 deletion leads to altered regulation of 935 genes in C. glabrata wild-type cells.

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Gene expression profiling of RPMI-grown and macrophage-internalized Candida glabrata wild-type, Cgrsc3-a∆ and Cgrtt109∆ cells.

Project description:To delineate the interaction of Candida glabrata with host immune cells, we performed genome-wide transcriptional profiling analysis on THP-1 macrophage-internalized wild-type and chromatin remodeling defective mutant (Cgrsc3-a∆ and Cgrtt109∆) yeasts. Genes implicated in ergosterol biosynthesis, and high-affinity iron uptake and homeostasis were found to be down-regulated in C. glabrata wild-type and mutant cells upon macrophage internalization. Additionally, global gene expression profiles of RPMI-grown and macrophage-ingested Cgrsc3-a∆ and Cgrtt109∆ cells revealed down-regulation of genes involved in mitochondrial respiration under normal growth conditions and induction of genes required for generation of precursors of metabolites and energy upon macrophage internalization. To examine the behavior of Candida glabrata wild-type and chromatin remodeling defective mutants upon internalization by differentiated human monocytic THP-1 cells, we compared the transcript profiles of 10 hour RPMI-grown with those of 10 hour THP-1 macrophage internalized C. glabrata cells. Additionally, early transcriptional response of C. glabrata wild-type cells to macrophage internal milieu was examined post 2 hour THP-1 macrophage infection.

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Gene expression profiling of RPMI-grown and macrophage-internalized Candida glabrata wild-type, Cgrsc3-aM-bM-^HM-^F and Cgrtt109M-bM-^HM-^F cells.

Project description:To delineate the interaction of Candida glabrata with host immune cells, we performed genome-wide transcriptional profiling analysis on THP-1 macrophage-internalized wild-type and chromatin remodeling defective mutant (Cgrsc3-aM-bM-^HM-^F and Cgrtt109M-bM-^HM-^F) yeasts. Genes implicated in ergosterol biosynthesis, and high-affinity iron uptake and homeostasis were found to be down-regulated in C. glabrata wild-type and mutant cells upon macrophage internalization. Additionally, global gene expression profiles of RPMI-grown and macrophage-ingested Cgrsc3-aM-bM-^HM-^F and Cgrtt109M-bM-^HM-^F cells revealed down-regulation of genes involved in mitochondrial respiration under normal growth conditions and induction of genes required for generation of precursors of metabolites and energy upon macrophage internalization. To examine the behavior of Candida glabrata wild-type and chromatin remodeling defective mutants upon internalization by differentiated human monocytic THP-1 cells, we compared the transcript profiles of 10 hour RPMI-grown with those of 10 hour THP-1 macrophage internalized C. glabrata cells. Additionally, early transcriptional response of C. glabrata wild-type cells to macrophage internal milieu was examined post 2 hour THP-1 macrophage infection. Agilent one-color experiment, Organism: Yeast (Candida glabrata)

2012-08-18 | E-GEOD-38953 | biostudies-arrayexpress

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Project description:Transcriptional profiling of Candida glabrata zap1Δ and zap1Δ::ZAP1 strains, comparing cells grown under planktonic and biofilm-inducing conditions. Goal was to analyse the effect of ZAP1 deletion in the transcriptomic profile of C. glabrata biofilm cells (in comparision to planktonic cells), under acidic conditions, in order to study the function of the Zap1 transcription factor in C. glabrata biofilm matrix production.

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Homo sapiens whole blood infected with Candida spp. (here: Candida glabrata)

Project description:Homo sapiens fresh whole blood was infected with Candida glabrata. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Candida glabrata gene expression.

2018-12-26 | GSE114175 | GEO

Limosilactobacillus fermentum Limits Candida glabrata Growth by Ergosterol Depletion

Project description:Candida glabrata is a human-associated opportunistic fungal pathogen. It shares its niche with Lactobacillus spp. in the gastrointestinal and vaginal tract. In fact, Lactobacillus species are thought to competitively prevent Candida overgrowth. We investigated the molecular aspects of this antifungal effect by analyzing the interaction of C. glabrata strains with Limosilactobacillus fermentum. From a collection of clinical C. glabrata isolates, we identified strains with different sensitivities to L. fermentum in coculture. We analyzed the variation of their expression pattern to isolate the specific response to L. fermentum. C. glabrata-L. fermentum coculture induced genes associated with ergosterol biosynthesis, weak acid stress, and drug/chemical stress. L. fermentum coculture depleted C. glabrata ergosterol. The reduction of ergosterol was dependent on the Lactobacillus species, even in coculture with different Candida species. We found a similar ergosterol-depleting effect with other lactobacillus strains (Lactobacillus crispatus and Lactobacillus rhamosus) on Candida albicans, Candida tropicalis, and Candida krusei. The addition of ergosterol improved C. glabrata growth in the coculture. Blocking ergosterol synthesis with fluconazole increased the susceptibility against L. fermentum, which was again mitigated by the addition of ergosterol. In accordance, a C. glabrata Derg11 mutant, defective in ergosterol biosynthesis, was highly sensitive to L. fermentum. In conclusion, our analysis indicates an unexpected direct function of ergosterol for C. glabrata proliferation in coculture with L. fermentum.

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