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Long Term Fertilizer Experiment

ABSTRACT: Long Term Fertilizer Experiment Raw sequence reads

PROVIDER: PRJNA982762 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
	SRR24905127_1.fastq.gz	Fastqsanger.gz
	SRR24905127_2.fastq.gz	Fastqsanger.gz
	SRR24905128_1.fastq.gz	Fastqsanger.gz
	SRR24905128_2.fastq.gz	Fastqsanger.gz
	SRR24905129_1.fastq.gz	Fastqsanger.gz

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MS profiling of the Long Term Evolution Experiment

Project description:The metabolome of a cell is the integration point of an organism's environment, genetics, and gene expression pattern. The metabolic phenotype can be under selection and is known to contribute to adaption. However, the metabolome's inherent networked and convoluted nature makes relating mutations, metabolic changes, and effects on fitness challenging. To overcome this challenge, we use the Long Term Evolution Experiment (LTEE) as a model to understand how mutations can transduce themselves through a cellular network, eventually affecting metabolism and perhaps fitness. We used mass-spectropscopy to broadly survey the metabolomes of both ancestors and all 12 evolved lines and combined this with genomic and expression data to suggest how mutations that alter specific reaction pathways, such as the biosynthesis of nicotinamide adenine dinucleotide, might increase fitness in the system. Our work brings the field closer to a complete genotype-phenotype map for the LTEE and a better understanding of how mutations might affect fitness through the metabolome. We used mass-spectroscopy to profile metabolic changes in the Long Term Evolution Experiment and link these change to upstream changes in gene expression and mutations.

2022-12-01 | ST002431 | MetabolomicsWorkbench

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