Project description:Recent reports of directed reprogramming have raised questions about the stability of cell lineages. Here, we have addressed this issue, focusing upon skin-derived precursors (SKPs), a dermally-derived precursor cell. We show by lineage tracing that murine SKPs from dorsal skin originate from mesenchymal and not neural crest-derived cells. These mesenchymally-derived SKPs can, without genetic manipulation, generate functional Schwann cells, a neural crest cell type, and are highly similar at the transcriptional level to Schwann cells isolated from the peripheral nerve. This is not a mouse-specific phenomenon, since human SKPs that are highly similar at the transcriptome level can be made from facial (neural crest-derived) and foreskin (mesodermally-derived) dermis, and the mesodermally-derived SKPs can make myelinating Schwann cells. Thus, non-neural crest-derived mesenchymal precursors can differentiate into bona fide peripheral glia in the absence of genetic manipulation, suggesting that developmentally-defined lineage boundaries are more flexible than widely thought. We obtained 3 independent samples of nerve Schwann cells, SKP-derived Schwann cells, and Dorsal Trunk SKPs, each, from adult SD rats. Primary cells were isolated and cultured, and RNA was collected from those cultured samples. RNA samples deriving from these cells were analyzed on the Affymetrix Rat Gene 1.0 ST Array.

2014-08-01 | E-GEOD-57505 | biostudies-arrayexpress

RNA Sequencing Facilitates Quantitative Analysis of Schwann cells Transcriptomes

Project description:Purpose: Whole-transcriptome sequencing technology and bioinformatics analysis were applied to systematically analyze the differentially expressed mRNAs, lncRNAs and miRNAs in SCs from DPN rats and control rats. Methods: mRNA, lncRNA and miRNA profiles of Schwann cells from DPN rats and control rats were generated by RNA sequencing, using Illumina Xten. The sequence reads that passed quality filters were analyzed at the transcript isoform level by using sequence analysis programs, including HISAT, Stringtie and DESeq2. qRT–PCR validation was performed using Takara, Vazyme and Clontech kits. Results: The data showed that 2925 mRNAs, 164 lncRNAs and 49 miRNAs were significantly differently expressed in SCs from DPN rats compared with control rats, with a fold change ≥1.2 or ≤ 0.833 and p value <0.05 . The results of qRT-PCR confirmed 13 mRNAs, 7 lncRNAs and 7 miRNAs which were consistent with the RNA-seq data. Functional and pathway analyses revealed that many enriched biological processes of GO terms and pathways were relevant to the function of SCs and the pathogenesis of DPN. Our study is the first to detect and analyze mRNAs, lncRNAs and miRNAs changes in Schwann cells from STZ-treated rats with DPN and reveal the novel interactions between dysregulated RNAs and the pathogenesis of DPN. Our data show that dysregulated RNAs have complicated interactions between them and play critical roles in regulating functions of SCs involved in the pathogenesis of DPN

2020-01-01 | GSE142785 | GEO

mRNA profiling of schwann cells cultured with monocytes derived macrophages and PDAC cell lines supernants-induced tumor-associated macrophages (TAMs)

Project description:Tumor associated macrophages(TAMs) have been demonstrated to promote tumor progression and perineural invasion in PDAC, however the underlying machanism of TAMs interacting with Schwann cells is still unclear. We found the gene expression of Schwann cell is different from TAMs-induced Schwann cells by comparing mRNA profiling.

2023-12-20 | GSE194205 | GEO

H9 human embryonic stem cells, Neural Precursor Cells

Project description:Identify gene expression patterns of different neural precursor cells derived from human embryonic stem cells.

2015-04-30 | GSE67383 | GEO

Embryonic stem cells exhibit mRNA isoform-specific translational regulation

Project description:Investigation of the differential translation rates of individual mRNA variants in embryonic stem cells (ESCs) and in ESC-derived neural precursor cells (NPCs) using polysome profiling coupled to RNA sequencing.

2016-01-26 | GSE73467 | GEO

RNA-seq transcriptional profiling in sciatic nerves from wildtype and Pmp22 transgenic rats during perinatal development

Project description:The goal of this study was to identify deregulated genes in Schwann cells of Pmp22 transgenic rats in comparison to wildtype rats. Three timepoints in the course of peripheral nerve myelination were chosen (embryonic day [E] 21, perinatal day [P]6 and P18) in order to reveal mechanistic insight into early pathological processes of Charcot-Marie-Tooth disease 1A (CMT1A).

2018-06-19 | GSE115930 | GEO

Schwann cell condition medium MS

Project description:The effects of Schwann cells on the neuro-stroma niche in pancreatic ductal adenocarcinoma (PDAC) remain to be explored. Here, single-cell RNA-sequencing and spatial transcriptome analysis of PDAC tissues reveals that Schwann cells induce malignant subtypes of tumour cells and cancer-associated fibroblasts. Mass Spectrometry (MS) were performed to detected the potential functional factors secreted by Schwann cells.

2023-06-27 | PXD042556 | Pride

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