Ontology highlight
ABSTRACT:
PROVIDER: PRJNA983964 | ENA |
REPOSITORIES: ENA
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Project description:Precious coral Corallium japonicum RAD-seq project
| PRJDB14374 | ENA
Project description:The process of calcium carbonate biomineralization has arisen multiple times during metazoan evolution. In the phylum Cnidaria, biomineralization has mostly been studied in the subclass Hexacorallia (i.e. stony corals) in comparison to the subclass Octocorallia (i.e. red corals); the two diverged approximately 600 million years ago. The precious Mediterranean red coral, Corallium rubrum, is an octocorallian species, which produces two distinct high-magnesium calcite biominerals, the axial skeleton and the sclerites. In order to gain insight into the red coral biomineralization process and cnidarian biomineralization evolution, we studied the protein repertoire forming the organic matrix (OM) of its two biominerals. We combined High-Resolution Mass Spectrometry and transcriptome analysis to study the OM composition of the axial skeleton and the sclerites. We identified a total of 102 OM proteins, 52 are shared between the two red coral biominerals with scleritin being the most abundant protein in each fraction. Contrary to reef building corals, the red coral is collagen-rich (10 collagen-like proteins). Agrin-like glycoproteins and proteins with sugar-binding domains are also predominant. Twenty-seven and 23 proteins were uniquely assigned to the axial skeleton and the sclerites, respectively. Their inferred regulatory function suggests that the difference between the two biominerals rather relies on the modeling of the matrix network than on specific structural components. At least one OM component appears to have been horizontally transferred from prokaryotes early during Octocorallia evolution. Our results support the view that calcification of the red coral axial skeleton likely represents a secondary calcification of an ancestral gorgonian horny axis. In addition, the comparison with stony coral skeletomes highlighted the low proportion of similar proteins between the biomineral OMs of hexacorallian and octocorallian corals, suggesting an independent acquisition of calcification in anthozoans.
2021-09-09 | PXD020332 | Pride
Project description:Corallium rubrum (red coral), genome assembly, jaCorRubr1.2
| PRJEB80373 | ENA
Project description:Corallium rubrum (Red Coral), genomic and transcriptomic data.
| PRJEB54015 | ENA
Project description:Soft corals are unique amongst animals in their prolific production of bioactive terpenoid natural products that rival the chemical diversity of plants and microbes. We recently established that octocorals uniformly express terpene cyclases and that their encoding genes often reside within putative biosynthetic gene clusters, a feature uncommon in animal genomes. In this work, we report the discovery and characterization of a widespread gene cluster family for the biosynthesis of briarane diterpenoids that number over 600 molecules distinct to corals. We sequenced five genomes from evolutionarily discrete families of briarane-producing octocorals, including the chromosomally resolved precious coral Corallium rubrum, and identified a common five-gene cluster composed of a terpene cyclase, three cytochrome P450s, and a short-chain dehydrogenase. Using Escherichia coli and Saccharomyces cerevisiae as hosts and homologous briarane biosynthesis genes from seven corals, we reconstituted the biosynthesis of cembrene B g-lactone, which contains the g-lactone structural feature distinctive of briarane diterpenoids. The discovery of the genomic basis of briarane biosynthesis not only allows for its biological examination across coral species but establishes that animals, like microbes and plants, also employ gene cluster families to produce specialized metabolites.
2024-05-17 | MSV000094792 | MassIVE