Project description:This study aimed to evaluate the gene expression signature of HSC-3 cells and its subline adapted to suspension culture (HSC-3-S5). Total RNAs were isolated from HSC-3 cells and HSC-3-S5 cells, and gene expression signatures were examined using gene expression microarray.
Project description:Metastasis to cervical lymph nodes of oral squamous cell carcinoma (OSCC) leads to a poor prognosis. The present study aimed at investigating the pathways and molecules associated with OSCC metastasis. The transcriptome between HSC-3 cells and their highly metastatic subline, HSC-3-M3 cells, was examined using gene expression microarray.
Project description:Hybrid sterility is a major form of postzygotic reproductive isolation, which is a widespread phenomenon in plants. Hybrids between two subspecies of the Asian cultivated rice (Oryza sativa L.), indica and japonica, are usually highly sterile. A killer-protector system composed of three closely linked genes, ORF3, ORF4 and ORF5 at the S5 locus, has been unveiled to regulate hybrid female fertility. We performed transcriptomic analysis of wild type Balilla, sterile BalillaORF5+ (Balilla with a transformed ORF5+) and fertility-restored BalillaORF3+ORF5+ (Balilla with a transformed ORF3+ and a transformed ORF5+) at S1 (before meiosis), S2 (during meiosis) and S3 (after meiosis) respectively. By comparing the differentially expressed genes in different comparisons, we proposed a model for the mechanisms of the S5 mediated hybrid sterility.
Project description:HSC-2 (hepatic stellate cells line from rat) were stably transfected with rno-miR-146a. Three different clones were selected (S1, S4, S5). We used Affymetrix rat genome RAT230 2.0 chip to monitor global transcriptome changes. Two passages of each sample were used. Control - HSC-2 and GFPscr.
Project description:HSC-2 (hepatic stellate cells line from rat) were stably transfected with rno-miR-146a. Three different clones were selected (S1, S4, S5). We used Affymetrix rat genome RAT230 2.0 chip to monitor global transcriptome changes.