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Epigenome analysis of control and mTORC2-suppressed glioblastoma cells

ABSTRACT: Epigenome analysis of control and mTORC2-suppressed glioblastoma cells

PROVIDER: PRJNA985551 | ENA |

REPOSITORIES: ENA

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Epigenome analysis of control and mTORC2-suppressed glioblastoma cells

Project description:Genome wide DNA methylation profiling of control and mTORC2-suppressed glioblastoma cells (U87-EGFRvIII cells). The Illumina Infinium HumanMethylation EPIC BeadChip Array was used to obtain DNA methylation profiles with 865,918 probes in glioblastoma cell line samples. Samples included 2 control U87-MG cells without mTORC2 suppresion, and mTORC2-knockdown U87-MG cells with lentivirus-mediated suppression of mTORC2.

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Project description:We herein demonstrate that mammalian target of rapamycin complex 2 (mTORC2), a critical core component of the growth factor signaling system, globally alters histone modification as well as transcriptome through metabolic reprogramming in the highly malignant brain tumor glioblastoma (GBM). Integrated analyses unravel that mTORC2 regulates mineral metabolism including iron trafficking via histone H3K9 acetylation of the ferritin promoter, facilitating GBM growth and survival.

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