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Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice

ABSTRACT: Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice

PROVIDER: PRJNA985562 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24967616_1.fastq.gz	Fastqsanger.gz
	SRR24967616_2.fastq.gz	Fastqsanger.gz
	SRR24967617_1.fastq.gz	Fastqsanger.gz
	SRR24967617_2.fastq.gz	Fastqsanger.gz
	SRR24967618_1.fastq.gz	Fastqsanger.gz

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Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [RNA-Seq]

2023-11-15 | GSE235213 | GEO

Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [MEDIP-seq]

Project description:Background: Neonicotinoids, a widely used class of insecticide that used is agriculture to control insect’s population, are serious public concern. Objectives: In this study, we examined the capacity of a neonicotinoid, thiacloprid (thia) to induce transgenerational effects in the male mice. Methods: The pregnant outbred Swiss female mice were exposed to thia at embryonic days E6.5 to E15.5 using “0”, and “6” mg/kg/day doses. The genome-wide analysis of DNA methylation analysis showed that differentially methylated regions (DMRs) are located at the promoters and enhancers of many important developmental genes. Notably, DMRs were largely overlapping the germ cell reprograming responsive gene (GRRs) and super-enhancer elements found to be active in ES cells, testis or brain.

2023-11-15 | GSE234861 | GEO

Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [MEDIP-seq]

Project description:Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [MEDIP-seq]

| PRJNA983361 | ENA

Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [RNA-Seq]

Project description:Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice [RNA-Seq]

| PRJNA985566 | ENA

Ancestral gestational exposure to widely used neonicotinoid thiacloprid leads to global DNA methylation alterations in spermatozoa in three generations of male mice

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-11-15 | GSE235215 | GEO

Molecular effects of neonicotinoid thiacloprid in honey bees (Apis mellifera)

Project description:Molecular effects of neonicotinoid thiacloprid in honey bees (Apis mellifera)

| PRJNA599219 | ENA

Use of the synergist piperonyl butoxide slows the development of insecticide resistance in the whitefly Bemisia tabaci

Project description:In the current study we examined the speed of resistance development in the silverleaf whitefly, Bemisia tabaci, after selection with either a neonicotinoid (thiacloprid) or pyrethroid (alpha-cypermethrin) insecticide alone or in combination with PBO. We used one control sample with no selection, 2 samples for neonicotinoid (thiacloprid) and Pyrethroid treatment and 2 samples for neonicotinoid (thiacloprid) + PBO and Pyrethroid + PBO treatments. The findings of this study demonstrate that PBO used in combination with certain insecticides can suppress the development of resistance in a laboratory setting.

2016-05-02 | E-MTAB-4601 | biostudies-arrayexpress

Identification of molecular mechanisms associated with resistance to the neonicotinoid insecticide imidacloprid in the dengue vector Aedes aegypti

Project description:This microarray study aimed at comparing constitutive gene expression levels between an Aedes aegypti insecticide-resistant strain (Imida-R) selected at the larval stage with the neonicotinoid insecticide imidacloprid for 10 generations and the parental strain (Bora-Bora) susceptible to all insecticides. Strains comparison was performed at both larval (4th stage larvae) and adult (3 days-old adults females, non-blood fed) stages.

2012-03-31 | E-MTAB-616 | biostudies-arrayexpress

Epigenetic Effects Promoted by Neonicotinoid Thiacloprid Exposure.

Project description:BackgroundNeonicotinoids, a widely used class of insecticide, have attracted much attention because of their widespread use that has resulted in the decline of the bee population. Accumulating evidence suggests potential animal and human exposure to neonicotinoids, which is a cause of public concern.ObjectivesIn this study, we examined the effects of a neonicotinoid, thiacloprid (thia), on the male reproductive system.MethodsThe pregnant outbred Swiss female mice were exposed to thia at embryonic days E6.5 to E15.5 using "0," "0.06," "0.6," and "6" mg/kg/day doses. Adult male progeny was analyzed for morphological and cytological defects in the testes using hematoxylin and eosin (H&E) staining. We also used immunofluorescence, Western blotting, RT-qPCR and RNA-seq techniques for the analyses of the effects of thia on testis.ResultsWe found that exposure to thia causes a decrease in spermatozoa at doses "0.6" and "6" and leads to telomere defects at all tested doses. At doses "0.6" and "6," thia exposure leads to an increase in meiotic pachytene cells and a decrease in lumen size, these changes were accompanied by increased testis-to-body weight ratios at high dose. By using RNA-seq approach we found that genes encoding translation, ATP production, ATP-dependent proteins and chromatin-modifying enzymes were deregulated in testes. In addition, we found that exposure to thia results in a decrease in H3K9me3 levels in spermatocytes. The changes in H3K9me3 were associated with a dramatic increase in activity of retroelements.ConclusionOur study suggests that gestational exposure to thia affects epigenetic mechanisms controlling meiosis which could lead to deleterious effects on male spermatogenesis.

| S-EPMC8290843 | biostudies-literature

Ancestral Folate Promotes Neuronal Regeneration in Serial Generations of Progeny [MeDIP]

Project description:Ancestral folate promotes neuronal regeneration in serial generations of progeny, eliciting transgenerational disruptions in DNA methylation.

2020-03-01 | GSE137642 | GEO

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