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Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [ChIP-Seq 2]

ABSTRACT: Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [ChIP-Seq 2]

PROVIDER: PRJNA985605 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24968141_1.fastq.gz	Fastqsanger.gz
	SRR24968141_2.fastq.gz	Fastqsanger.gz
	SRR24968142_1.fastq.gz	Fastqsanger.gz
	SRR24968142_2.fastq.gz	Fastqsanger.gz
	SRR24968143.fastq.gz	Fastqsanger.gz

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Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [RNA-seq]

Project description:Members of the NSL histone acetyltransferase complex are involved in multi-organ developmental syndromes. While the NSL complex is known for its importance in early development, its role in fully differentiated cells remains enigmatic. Using a kidney-specific model, we discovered that deletion of NSL complex members KANSL2 or KANSL3 in post-mitotic podocytes led to catastrophic kidney dysfunction. A systematic comparison of two primary differentiated cell types revealed that the NSL complex is a master regulator of intraciliary transport genes in both dividing and non-dividing cells. NSL complex ablation led to loss of cilia and impaired sonic hedgehog pathway in ciliated fibroblasts. By contrast, non-ciliated podocytes responded with altered microtubule dynamics and obliterated podocyte functions. Finally, overexpression of wild type but not a double zinc-finger (ZF-ZF)-domain mutant of KANSL2 rescued the transcriptional defects, revealing a critical function of this domain in NSL complex assembly and function. Thus, the NSL complex targets exhibit bifurcation of functions to enable diversity of specialized outcomes in differentiated cells.

2023-07-31 | GSE225433 | GEO

Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [ChIP-seq]

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Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [ChIP-Seq 2]

2023-07-31 | GSE235243 | GEO

Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells.

Project description:Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells.

| PRJNA935690 | ENA

Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [RNA-seq]

Project description:Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [RNA-seq]

| PRJNA935696 | ENA

Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [ChIP-seq]

Project description:Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells [ChIP-seq]

| PRJNA935697 | ENA

Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus nonciliated cells.

Project description:Members of the NSL histone acetyltransferase complex are involved in multiorgan developmental syndromes. While the NSL complex is known for its importance in early development, its role in fully differentiated cells remains enigmatic. Using a kidney-specific model, we discovered that deletion of NSL complex members KANSL2 or KANSL3 in postmitotic podocytes led to catastrophic kidney dysfunction. Systematic comparison of two primary differentiated cell types reveals the NSL complex as a master regulator of intraciliary transport genes in both dividing and nondividing cells. NSL complex ablation led to loss of cilia and impaired sonic hedgehog pathway in ciliated fibroblasts. By contrast, nonciliated podocytes responded with altered microtubule dynamics and obliterated podocyte functions. Finally, overexpression of wild-type but not a double zinc finger (ZF-ZF) domain mutant of KANSL2 rescued the transcriptional defects, revealing a critical function of this domain in NSL complex assembly and function. Thus, the NSL complex exhibits bifurcation of functions to enable diversity of specialized outcomes in differentiated cells.

| S-EPMC10456878 | biostudies-literature

Transcriptional regulation by the NSL complex enables diversification of IFT functions in ciliated versus non-ciliated cells.

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-07-31 | GSE225434 | GEO

The NSL complex–mediated nucleosome landscape is required to maintain transcription fidelity and suppression of transcription noise

Project description:We report that the Drosophila Non-Specific Lethal (NSL) complex is necessary to maintain this stereotypical nucleosomal organization at promoters. Upon NSL1 depletion, nucleosomes invade the NDRs at TSSs of NSL-bound genes. NSL complex member NSL3 binds to TATA-less promoters in a sequence-dependent manner. We showed that the NSL complex is necessary and sufficient to recruit NURF chromatin remodeling complex to target promoters. The NSL complex is not only essential for transcription but is required for accurate TSS selection for genes with multiple TSSs. Further, loss of NSL complex leads to an increase in transcriptional noise. Thus, the NSL complex establishes a canonical nucleosomal organization that enables transcription and determines TSS fidelity.

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Analysis of NSL knockout brains

Project description:In the current study, we analyse the function of MOF-NSL complex in neural cells. We find that the NSL complex regulates metabolic networks in the brain.

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