Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [CUT&RUN]

ABSTRACT: CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [CUT&RUN]

PROVIDER: PRJNA985679 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR24974363_1.fastq.gz	Fastqsanger.gz
	SRR24974363_2.fastq.gz	Fastqsanger.gz
	SRR24974364_1.fastq.gz	Fastqsanger.gz
	SRR24974364_2.fastq.gz	Fastqsanger.gz
	SRR24974365_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [CUT&RUN]

Project description:CUX1 is a HOX-family transcription factor that is essential for development and differentiation of multiple tissues. CUX1 is recurrently mutated or deleted in cancer, particularly in myeloid malignancies. However, the mechanism by which CUX1 regulates gene expression and differentiation remains poorly understood, creating a barrier to understanding the tumor suppressive functions of CUX1. Herein, we demonstrate that CUX1 directs the BAF chromatin remodeling complex to DNA to increase chromatin accessibility in hematopoietic cells. CUX1 preferentially regulates lineage-specific enhancers, and CUX1 target genes are predictive of cell fate in vivo. These data indicate that CUX1 functions as a pioneer factor to epigenetically regulate hematopoietic lineage commitment and homeostasis, and CUX1 deficiency disrupts these processes in stem and progenitor cells, facilitating transformation.

2023-10-01 | GSE235303 | GEO

CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [ChIP-seq]

2023-10-01 | GSE235301 | GEO

CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [ATAC-seq]

2023-10-01 | GSE235299 | GEO

CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex

Project description:CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex

| PRJNA985668 | ENA

CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [ATAC-seq]

Project description:CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [ATAC-seq]

| PRJNA985674 | ENA

CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [ChIP-seq]

Project description:CUX1 regulates human hematopoietic stem cell chromatin accessibility via the BAF complex [ChIP-seq]

| PRJNA985672 | ENA

Acute BAF perturbation causes immediate changes in chromatin accessibility

Project description:Loss-of-function mutations in genes coding for subunits of the large, multifarious BRG1/BRM associated factor (BAF) chromatin remodeling complexes are frequently causative for cancer or developmental diseases1-5. Cells lacking the most frequently mutated subunits like the ATPase SMARCA4 typically exhibit drastic chromatin accessibility changes, especially of important regulatory regions6-12. However, so far it remains unknown how these changes are established over time, and whether they are causative for intra-complex synthetic lethalities abrogating the formation (SMARCC1-SMARCC2)8,13,14 or activity (SMARCA4-SMARCA2)15-17 of BAF complexes. Here, we utilize the dTAG system18 to induce acute degradation of BAF subunits in wild-type and BAF mutant backgrounds and analyze the resulting chromatin accessibility changes with high kinetic resolution. We observe that chromatin alterations are established faster than the duration of one cell cycle and that maintaining genome accessibility requires constant ATP-dependent remodeling. Completely abolishing BAF complex function by acute degradation of a synthetic lethal subunit in a paralog-deficient background results in a near-complete loss of chromatin accessibility at BAF-controlled sites, especially at super-enhancers, providing a mechanism for intra-complex synthetic lethalities.

2020-12-23 | PXD018381 | Pride

The BAF complex does not mediate GLI repression (WT Cut&RUN)

Project description:The BAF complex does not mediate GLI repression (WT Cut&RUN)

| PRJNA934436 | ENA

The BAF complex does not mediate GLI repression (Gli3 null Cut&RUN)

Project description:The BAF complex does not mediate GLI repression (Gli3 null Cut&RUN)

| PRJNA934435 | ENA

Bulk Cut&run and Cut&tag

Project description:Bulk Cut&run and Cut&tag

| PRJNA686390 | ENA