Project description:Analysis of Arabidopsis Columbia and rve8-1, a longer period mutant, in circadian time course (ZT72-116). Results provide insight into where RVE8 functions in the clock system and the targets regulated by RVE8.
Project description:Interaction proteomics time course over 24h every 4h, looking for novel interactors of the circadian clock and flowering time protein GIGANTEA. Plants expressing 35S:GIGANTEA:3xFlag6His were used, as well as WT plants for a background control.
Project description:We generated a tamoxifen-inducible skeletal muscle-specific Bmal1 knockout mouse model and performed a time-course microarray experiment to identify gene expression changes downstream of the molecular clock. We report the transcrpt expression profiles in adult gastrocnemius skeletal muscle harvested from iMS-Bmal1-/- and at 4 hour intervals for a full circadian time-course (6 time-points).
Project description:Analysis of Arabidopsis Columbia and rve8-1, a longer period mutant, in circadian time course (ZT72-116). Results provide insight into where RVE8 functions in the clock system and the targets regulated by RVE8. Arabidopsis Col and rve8-1 seedlings (~50 plants/ per sample) were grown in 12 hours light/ 12 hours dark for 7 days before releasing to the constant light. After 72 hours in the constant light, samples were harvested every 4 hours for 2 days. RNA was extracted, labeled and hybridized onto Agronomics1 tiling arrays.
Project description:Botryococcus braunii is a colony forming green microalga of the order Chlorophyta. During the growth cycle of this organism, the algae synthesizes long chain liquid hydrocarbon isoprenoid compounds and sequesters them in the extracellular matrix of the colony. Metabolomics was done on samples from a circadian time series.
The work (proposal:https://doi.org/10.46936/10.25585/60000723) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231.
Project description:A 30-h time-course RNA-seq study was performed to analyse the different circadian phenotypes of SW480 and SW620 cells, a cell line from a primary tumour and a metastatic cell line from the same patient. Samples were taken every 3 h for a period of 30 h resulting in 11 time-points for each cell line.
