RNA Bind-N-Seq for purified α-Synuclein and BG4 (scFV for G-quadruplex)
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA986023 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR24977861.fastq.gz | Fastqsanger.gz | |||
| SRR24977862.fastq.gz | Fastqsanger.gz | |||
| SRR24977863.fastq.gz | Fastqsanger.gz | |||
| SRR24977864.fastq.gz | Fastqsanger.gz | |||
| SRR24977865.fastq.gz | Fastqsanger.gz |
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Project description:Aggregation of α-synuclein may trigger the development of synucleinopathies, a progressive neurodegenerative disease such as Parkinson's disease and Dementia with Lewy bodies. We demonstrate RNA initiates α-synuclein phase transition in vitro, however, RNA binding ability and its motif remain unclear. Here, we indicate purified human α-synuclein protein preferentially binds to guanine-rich RNA sequences by RNA Bind-N-Seq using a pool of random 24-mer RNA oligonucleotides in vitro. Importantly, these hit motifs were required consecutive guanines not random, suggesting that RNA G-quadruplexes binds to α-synuclein. We also confirmed that GC content and skew of α-synuclein-enriched RNA sequences were similar to that of BG4 (G-quadruplex antibody)-enriched RNA sequences. Taken together, these data suggest that α-synuclein binds to RNA-formed G-quadruplex.
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