Ontology highlight
ABSTRACT:
PROVIDER: PRJNA986075 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
2013-11-01 | GSE42557 | GEO
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
2013-11-01 | GSE42556 | GEO
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
2013-11-01 | GSE42555 | GEO
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature. Ninety-eight samples from three separate temperature exposure studies were analyzed on ninety-eight microarrays, using a common reference design, with multiple biological replicates for each temperature condition for each year of the experiment.
2013-11-01 | E-GEOD-42555 | biostudies-arrayexpress
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature. Ninety-eight samples from three separate temperature exposure studies were analyzed on ninety-eight microarrays, using a common reference design, with multiple biological replicates for each temperature condition for each year of the experiment.
2013-11-01 | E-GEOD-42556 | biostudies-arrayexpress
Project description:A large-scale functional genomics study revealed shifting energy generating processes in white muscle during the final 1,300 km migration of wild sockeye salmon to their spawning grounds in the Fraser River, British Columbia. In 2006, Lower Adams stock sockeye salmon ceased feeding after passing the Queen Charlotte Islands, 850 km from the Fraser River. Enhanced protein turnover and reduced transcription of actin, muscle contractile and heme-related proteins were early starvation responses in saltwater. Arrival to the estuarine environment triggered massive protein turnover through induction of proteasoma and lysosomal proteolysis and protein biosynthesis, and a shift from anaerobic glycolysis to oxidative phosphorylation. Response to entry into freshwater was modest, with up-regulation of heat shock proteins and nitric oxide biosynthesis. High river temperatures resulted in a strong defense/immune response and high mortalities in 50% of fish. Arrival to the spawning grounds triggered further up-regulation of oxidative phosphorylation and proteolysis, down-regulation of protein biosynthesis and helicase activity, and continued down-regulation of muscle proteins and most glycolytic enzymes. However, sharp up-regulation of PFK-I indicated induction of glycolytic potential at the spawning grounds. The identification of potential environmental cues triggering genome-wide transcriptional shifts in white muscle associated with migration and the strong activation of proteasomal proteolysis were both novel findings. Keywords: Functional genomics study on wild-caught fish
2008-12-09 | GSE13657 | GEO
Project description:We collected sockeye salmon from the Fraser River, British Columbia, and held them at ecologically relevant temperatures (14C and 19C) determine the effect of elevated water temperature on cellular processes in non-lethally sampled gill tissue and blood plasma over a period of seven days that represents a significant portion of their upstream migration. Time-matched fish that died prematurely over the course of the holding study were also sampled for gill tissue and the transcriptomic responses in moribund fish were compared with surviving fish. This is the first study to experimentally examine transcriptomic responses to high water temperature and premature mortality in wild-caught Pacific salmon and the results will help in understanding some of the cellular mechanisms involved in large-scale migration mortality in Pacific salmon during warm water periods and for predicting or understanding causes of mortality in naturally senescing adult Pacific salmon.
2012-05-31 | GSE33586 | GEO
Project description:We collected sockeye salmon from the Fraser River, British Columbia, and held them at ecologically relevant temperatures (14C and 19C) determine the effect of elevated water temperature on cellular processes in non-lethally sampled gill tissue and blood plasma over a period of seven days that represents a significant portion of their upstream migration. Time-matched fish that died prematurely over the course of the holding study were also sampled for gill tissue and the transcriptomic responses in moribund fish were compared with surviving fish. This is the first study to experimentally examine transcriptomic responses to high water temperature and premature mortality in wild-caught Pacific salmon and the results will help in understanding some of the cellular mechanisms involved in large-scale migration mortality in Pacific salmon during warm water periods and for predicting or understanding causes of mortality in naturally senescing adult Pacific salmon. Forty samples were analyzed on forty two-channel microarrays, using a common reference design, with multiple biological replicates for each temperature condition. Fish were further classified into survivor and moribund based on their status after 7 days in captivity.
2012-05-30 | E-GEOD-33586 | biostudies-arrayexpress