Project description:This study was designed to look for differential gene expression in the annual dicot weed velvetleaf when it is grown in competition with corn relative to when it is grown in monoculture. Keywords: weed/crop competition
Project description:L-Lactic acid is a key monomer for polylactic acid (PLA), and its market demand continues to grow. Producing lactic acid from lignocellulosic feedstocks instead of grain can alleviate competition for food resources; however, inhibitors generated during pretreatment result in low fermentation titers and complex downstream processes, hindering industrial implementation. In this study, Pediococcus acidilactici NHRI04 with robust inhibitor tolerance was obtained through staged adaptive domestication, enabling direct fermentation of undetoxified corn stover hydrolysate. Process optimization achieved a final L-lactic acid concentration of 226.09 g/L, with a productivity of 3.12 g/L/h and a sugar-to-lactic acid yield of 99.72%, corresponding to an overall yield of 0.32 g/g corn stover and 98.80% optical purity. This titer represents the highest lactic acid concentration reported to date from lignocellulosic biomass. A pasteurization-based fermentation strategy (65 °C, 30 min) was established to replace conventional autoclaving, effectively preserving medium nutritional value. Economic analysis revealed that combining high-concentration fermentation, pasteurization, and detoxification elimination reduced production costs by 2,232 CNY per ton of lactic acid. Substituting corn stover for corn grain saves 1.8 tons of corn and reduces carbon emissions by 1.83 tons CO₂e per ton of lactic acid. Integrated whole-genome, transcriptomic, and proteomic analyses elucidated the molecular mechanisms underlying inhibitor tolerance. This study provides a feasible approach for low-cost, grain-saving, and low-carbon production of lignocellulosic lactic acid.
| PXD080923 |
Project description:Poecilia reticulata competition and salinity study
| PRJNA1334411 | ENA
Project description:study on corn oil supplementation
Project description:The purpose of this study was to explore the mechanism of aerobic decay of whole-plant corn silage and the effect of Neolamarckia cadamba essential oil on aerobic stability of whole-plant corn silage. Firstly, the dynamic changes of temperature, microbial community and metabolite content after aerobic exposure of whole-plant corn silage were determined, and the main microbial species and mechanism leading to aerobic spoilage of whole-plant corn silage were analyzed. The N. cadamba essential oil was extracted from fresh N. cadamba leaves by steam distillation, and the minimal inhibitory concentration, antibacterial stability and bacteriostatic mechanism of N. cadamba essential oil against undesirable microorganisms in whole-plant corn silage were determined. According to the minimum inhibitory concentration of N. cadamba essential oil on undesirable microorganisms in silage, N. cadamba essential oil was added to whole-plant corn silage to explore the effect of N. cadamba essential oil on the aerobic stability of whole-plant corn silage.