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Xrn1-sensitive lncRNAs are insensitive to Dbp2 nuclear depletion

ABSTRACT: Xrn1-sensitive lncRNAs are insensitive to Dbp2 nuclear depletion

PROVIDER: PRJNA986458 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR24991602_1.fastq.gz	Fastqsanger.gz
	SRR24991602_2.fastq.gz	Fastqsanger.gz
	SRR24991603_1.fastq.gz	Fastqsanger.gz
	SRR24991603_2.fastq.gz	Fastqsanger.gz
	SRR24991604_1.fastq.gz	Fastqsanger.gz

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Xrn1-sensitive lncRNAs are insensitive to Dbp2 nuclear depletion

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Xrn1-sensitive lncRNAs accumulate upon inactivation of Dbp2 and Mtr4

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Xrn1-sensitive lncRNAs accumulate upon inactivation of Dbp2 and Mtr4

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Translational landscape of Xrn1-sensitive lncRNAs in yeast

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Xrn1-sensitive lncRNAs are globally insensitive to the cytoplasmic helicases Ecm32, Ski2, Slh1, Dbp1 and Dhh1

Project description:Xrn1-sensitive lncRNAs are globally insensitive to the cytoplasmic helicases Ecm32, Ski2, Slh1, Dbp1 and Dhh1

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Nonsense-Mediated Decay restricts lncRNAs levels in yeast unless blocked by double-stranded RNA structure

Project description:Antisense long non-coding (aslnc)RNAs represent a substantial part of eukaryotic transcriptomes that are, in yeast, controlled by the Xrn1 exonuclease. Nonsense-Mediated Decay (NMD) destabilizes the Xrn1-sensitive aslncRNAs (XUT), but what determines their sensitivity remains unclear. We report that 3’ single-stranded (3’-ss) extension mediates XUTs degradation by NMD, assisted by the Mtr4 and Dbp2 helicases. Single-gene investigation, genome-wide RNA analyses and double-stranded (ds)RNA mapping revealed that 3'-ss extensions discriminate the NMD-targeted XUTs from stable lncRNAs. Ribosome profiling showed that XUT are translated locking them for NMD activity. Interestingly, mutants of the Mtr4 and Dbp2 helicases accumulated XUTs, suggesting that dsRNA unwinding is a critical step for degradation. Indeed, expression of anti-complementary transcripts protects cryptic intergenic lncRNAs from NMD. Our results indicate that aslncRNAs form dsRNA that are only translated and targeted to NMD if dissociated by Mtr4 and Dbp2. We propose that NMD buffers genome expression by discarding pervasive regulatory transcripts.

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The RNA helicases Dbp2 and Mtr4 regulate the expression of Xrn1-sensitive long non-coding RNAs in yeast

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