Project description:The aim of this study is to investigate whether the known mechanisms of hyperaccumulaion in Brassicaceae hyperaccumulators (Arabidopsis halleri and Noccaea caerulescens) are conserved among different hyperaccumulating species or if there exist unknown mechanismsin other metal hyperaccumulating species.The comparison of transcriptomes between hyperaccumulator Sedum plumbizincicola and non-hyperaccumulator S. alfredii (NHE) was performed by taking advantage of next-generation sequencing.The data suggested that increased active uptake, long-distance transport and efficient chelation of heavy metals might represent common mechanisms occurred in various hyperaccumulators. Moreover, we found that those genes showing constitutively higher expression in S. plumbizincicola shoots are significantly enriched in processes related to cell wall metabolism, including biosynthesis/modification of cellulose, lignin, pectin, glucan, and other cell wall related components.
Project description:Sedum plumbizincicola is an herbaceous species tolerant of excessive cadmium accumulation in above-ground tissues. The implications of membrane proteins, especially integrative membrane proteins such as transporters, in Cd detoxification in plants have received attention in recent years, but a comprehensive profiling of Cd-responsive membrane proteins from Cd hyperaccumulator plants is lacking. In this study, the membrane proteins of root, stem, and leaf tissues of S. plumbizincicola seedlings treated with Cd solution for 0, 1 or 3 days were analyzed by Tandem Mass Tag (TMT) labeling-based proteome quantification. Total 3353 proteins with predicted transmembrane helices were identified and quantified in at least one tissue group
