Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole transcriptome analysis of EBV infected peripheral blood mononuclear cells (PBMCs)

ABSTRACT: Whole transcriptome analysis of EBV infected peripheral blood mononuclear cells (PBMCs)

PROVIDER: PRJNA988068 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Whole transcriptome analysis of EBV infected peripheral blood mononuclear cells (PBMCs)

Project description:In order to gain insights into the intricate molecular alterations taking place following Epstein-Barr virus (EBV) infection in primary B-cells, we employed RNA-Seq to investigate the dynamic changes occurring within the cell transcriptome during the early stages of EBV infection (0, 2, and 4 days post infection). By analysing the transcriptomic data generated, our objective was to find novel pathways and mechanisms that may play pivotal roles in the EBV-induced transformation of B-cells, particularly during early stage of B-cell activation.

2023-08-03 | GSE235941 | GEO

miR from peripheral blood mononuclear cells' (PBMCs') exosome

Project description:miR from peripheral blood mononuclear cells' (PBMCs') exosome

| PRJNA786954 | ENA

Expression data for human peripheral blood mononuclear cells (PBMCs) transfected with EBV Bart 6-3p mimics.

Project description:Epstein-Barr virus (EBV) is highly successful virus infecting the majority of the human population worldwide. During the latent infection period, there are only a few of EBV-encoded proteins can be detected, whereas EBV-encoded non-coding RNAs are highly activated, especially microRNAs. Recent studies found that those BART microRNAs not only disturb EBV genes expression, but also interfere many host genes expression, thus modulating cellular proliferation and immune regulation. In the present study, we investigate the effect of EBV BART6-3p on gene expression profile of the human PBMCs.

2018-07-31 | GSE94312 | GEO

Differenatiation of miRNA expression in PBMCs (peripheral blood mononuclear cells)

Project description:Differenatiation of miRNA expression in PBMCs (peripheral blood mononuclear cells)

| PRJNA550408 | ENA

Goat Peripheral Blood Mononuclear Cells (PBMCs) responses to infection vary with Peste des Petits Ruminants Virus virulence

Project description:Peste des Petits Ruminants (PPR) is a highly infectious disease caused by a virus of the genus Morbillivirus (PPRV), infecting mainly sheep and goat. Susceptibility of host can vary widely with host breed and virus strain. The mechanisms underlying this variability are not well understood. Here, we carried out the first comparative in vitro study on goat peripheral blood mononuclear cells (PBMCs) infected with PPRV strains of different virulence, vaccine strain (N75/1), low- (IC89), and high-virulent strain (MA08). Goat PBMCs were infected by the different strains and stimulated with Concanavalin A (Con A), and proteome changes of these cells were evaluated during the infection. The level of PPRV replication was assessed first by RT-qPCR quantification of viral N mRNA and by labelling the viral N protein inside the cells with specific antibodies and analysed by flow cytometry. The dynamics of PBMC sub-populations were also evaluated by flow cytometry. Our results showed that viral replication is critical for PPRV inhibitory effect on PBMCs proliferation. Highly virulent MA08 strain reached a higher level of replication in ConA-stimulated PBMCs and induced higher mortality compared to other strains. Low-virulent IC89 strain showed the lower replication level and cell proliferative inhibitory capacities. Differences in immune genes expression levels were assessed using RNAseq analysis and protein expression was compared using mass spectrometry. Analysis of these data provided the transcriptional and proteome landscape of PBMCs infected with these strains. The possible association between the transcriptional and proteome landscape and changes in immune cell subpopulations dynamics was explored.

2021-11-03 | PXD024890 | Pride

Signature of circular RNAs in peripheral blood mononuclear cells (PBMCs) from patients with active tuberculosis

Project description:The knowledge of circRNAs in tuberculosis (TB) remains limited. Aim of the present study was to characterize the expression profiles and potential function of circRNAs in human peripheral blood mononuclear cells (PBMCs) from patients with active TB.

2018-12-29 | GSE117563 | GEO

Effect of Epstein-Barr Virus DNA on the Transcriptional Profile of BALB/c Mouse Peripheral Blood Mononuclear Cells

Project description:To examine the effect of DNA from the Epstein-Barr Virus (EBV) on the transcriptional profile of mouse peripheral blood mononuclear cells (PBMCs), BALB/c mouse cells were isolated and cultured in the presence or absence of EBV DNA. RNA was extracted from these cells and then subjected to sequencing. We observed significantly enhanced expression of genes that play roles in proinflammatory processes including ones involved in autoimmune diseases such as rheumatoid arthritis.

2024-04-09 | GSE171757 | GEO

Microarray analysis of peripheral blood mononuclear cells (PBMCs) from IgA nephropathy patients, membranous nephropathy patients and healthy controls.

Project description:To search for new markers of active lesions that might help better understand the molecular basis of MN and IgAN and aid in their diagnosis, DNA microarray analysis was performed with peripheral blood mononuclear cells (PBMCs)

2016-04-22 | GSE73953 | GEO

Focused transcription profiling of peripheral blood mononuclear cells from vasculitis patients

Project description:Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles.

2010-11-10 | GSE18247 | GEO

Comparative transcription profiling of peripheral blood mononuclear cells from vasculitis patients

Project description:Peripheral blood mononuclear cells (PBMCs) from 13 vasculitis patients expressed varying gene profiles.

2010-11-10 | GSE16945 | GEO