Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Enterobacter cloacae subsp. cloacae ATCC 13047

ABSTRACT: Enterobacter cloacae gentamicin heteroresistance

PROVIDER: PRJNA988129 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25054839.fastq.gz	Fastqsanger.gz
	SRR25054840.fastq.gz	Fastqsanger.gz
	SRR25054841.fastq.gz	Fastqsanger.gz

Items per page:

1 - 3 of 3

Similar Datasets

Aminoglycoside heteroresistance in Enterobacter cloacae is driven by the cell envelope stress response.

Project description:Enterobacter cloacae is a Gram-negative nosocomial pathogen of the ESKAPE (Enterococcus, Staphylococcus, Klebsiella, Acinetobacter, Pseudomonas, and Enterobacter spp.) priority group with increasing multi-drug resistance via the acquisition of resistance plasmids. However, E. cloacae can also display forms of antibiotic refractoriness, such as heteroresistance and tolerance. Here, we report that E. cloacae displays transient heteroresistance to aminoglycosides, which is accompanied with the formation of small colony variants (SCVs) with increased minimum inhibitor concentration (MIC) of gentamicin and other aminoglycosides used in the clinic, but not other antibiotic classes. To explore the underlying mechanisms, we performed RNA sequencing of heteroresistant bacteria, which revealed global gene-expression changes and a signature of the CpxRA cell envelope stress response. Deletion of the cpxRA two-component system abrogated aminoglycoside heteroresistance and SCV formation, pointing to its indispensable role in these processes. The introduction of a constitutively active allele of cpxA led to high aminoglycoside MICs, consistent with cell envelope stress response driving these behaviours in E. cloacae. Cell envelope stress can be caused by environmental cues, including heavy metals. Indeed, bacterial exposure to copper increased gentamicin MIC in the wild-type, but not in the ΔcpxRA mutant. Moreover, copper exposure also elevated the gentamicin MICs of clinical isolates from bloodstream infections, suggesting that CpxRA- and copper-dependent aminoglycoside resistance is broadly conserved in E. cloacae strains. Altogether, we establish that E. cloacae relies on transcriptional reprogramming via the envelope stress response pathway for transient resistance to a major class of frontline antibiotic.

2023-10-18 | GSE236124 | GEO

Enterobacter cloacae subsp. cloacae ATCC 13047

Project description:Enterobacter cloacae subsp. cloacae ATCC 13047

| PRJNA627053 | ENA

Enterobacter cloacae subsp. cloacae ATCC 13047

Project description:Enterobacter cloacae subsp. cloacae ATCC 13047 Genome sequencing

| PRJNA45793 | ENA

Enterobacter cloacae subsp. cloacae ATCC 13047

Project description:Enterobacter cloacae subsp. cloacae ATCC 13047 genome sequencing

| PRJNA59553 | ENA

Enterobacter cloacae subsp. cloacae ATCC 13047

Project description:Enterobacter cloacae subsp. cloacae ATCC 13047 Genome sequencing

| PRJNA992890 | ENA

Complete genome sequence of Enterobacter cloacae subsp. cloacae type strain ATCC 13047.

Project description:Enterobacter cloacae is an important nosocomial pathogen. Here, we report the completion of the genome sequence of E. cloacae ATCC 13047, the type strain of E. cloacae subsp. cloacae. Multiple sets of virulence determinant and heavy-metal resistance genes have been found in the genome. To the best of our knowledge, this is the first complete genome sequence of the E. cloacae species.

| S-EPMC2863489 | biostudies-literature

Heteroresistance in Enterobacter cloacae complex caused by cell-to-cell variation in transient gene duplication-amplification events

Project description:Heteroresistance in bacteria describes a subpopulational phenomenon of transient antibiotic resistance variation among cells of a generally susceptible population. Here, we investigated the molecular mechanisms and phenotypic characteristics underlying heteroresistance to ceftazidime (CAZ) in a clinical Enterobacter cloacae complex strain (ECC). We identified a plasmid-borne gene duplication-amplification (GDA) event of a region harboring an ampC gene encoding a β-lactamase blaDHA-1 as the key determinant of heteroresistance. Individual colonies exhibited variations in the copy number of the genes resulting in resistance level variation which correlated with growth onset (lag times) and growth rates in the presence of CAZ, analysed in linear models. GDA copy number heterogeneity occurred within single resistant colonies, demonstrating heterogeneity of GDA on the single-cell level. The interdependence between GDA, lag time and antibiotic treatment and the strong plasticity underlying heteroresistance underlines the high risk for misdetection of antimicrobial heteroresistance and subsequent treatment failure.

2024-10-17 | PXD056728 | Pride

Enterobacter cloacae strain:ATCC 13047

Project description:Enterobacter cloacae strain:ATCC 13047 Genome sequencing and assembly

| PRJNA244549 | ENA

Enterobacter cloacae subsp. cloacae

Project description:Enterobacter cloacae subsp. cloacae Genome sequencing

| PRJNA234334 | ENA

Proteomics analysis of the Enterobacter cloacae response to imipenem

Project description:Recently, we have reported on a highly drug-resistant carbapenemase-producing isolate of Enterobacter cloacae (Nepal et al., Virulence. 2018; 9: 1377-1389). In the present study, we asked the question whether and, if so, how this isolate responds to a sub-inhibitory challenge with the antibiotic imipenem. To answer this question, we applied a SILAC proteomics approach that allowed the quantification of changes in the relative abundance of bacterial protein in response to imipenem. The results show that the investigated E. cloacae isolate mounts a highly specific response to counteract the detrimental effects of imipenem.

2021-08-10 | PXD013412 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data