Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from cultured spermatogonia established from 10 day old mouse testes

ABSTRACT: Expression data from cultured spermatogonia established from 10 day old mouse testes

PROVIDER: PRJNA989091 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR25069759.fastq.gz	Fastqsanger.gz
	SRR25069760.fastq.gz	Fastqsanger.gz
	SRR25069761.fastq.gz	Fastqsanger.gz
	SRR25069762.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

Expression data from cultured spermatogonia established from 10 day old mouse testes

Project description:Spermatogonial stem cells (SSCs) undergo self-renewal division to sustain spermatogenesis. Although it is possible to derive germline stem (GS) cell cultures from most of the mouse strains by supplementing GDNF and FGF2, SSCs from a 129 background do not proliferate under the same culture conditions, which suggested that they have distinct self-renewal requirements. We modified previous culture conditions and established long-term culture of SSCs of 129 mice. 129 GS cells reinitiated spermatogenesis and produced offspring following transplantation into the seminiferous tubules of infertile mouse recipients. This dataset show the differences of gene expressions of GS cells between C57BL/6 and 129 mice, which have important implications in understanding requirements of self-renewal mechanisms. In this dataset, we include the expression data obtained from cultured spermatogonia (GS cells) derived from C57BL/6, and 129 mice. Each group contains 2 biological replicates.

2024-06-17 | GSE236119 | GEO

Expression data from 15-day-old mouse testes

Project description:Expression data from 15-day-old mouse testes

| PRJNA529660 | ENA

Expression data from spermatogonia enriched from 12 dpc mouse testes

Project description:Spermatogonial stem cells (SSCs) cotinues to produce sperm throughout life by self-renewal division. Although it is possible to expand SSCs from most of the mouse strains in vitro for a long-term period by supplementing GDNF and FGF2, SSCs from a 129 background do not proliferate under the same culture conditions. Since it suggested that they have distinct self-renewal requirements, gene expressions of spermatogonia enriched from testes of DBA/2, C57BL/6, and 129 strains were compared. In this dataset, we include the expression data obtained from spermatogonia enriched from 12dpc testes of DBA/2, C57BL/6, and 129. Enrichment was performed by magnetic activated-cell sorting using anti-Ecadherin (CDH1) antibody (ECCD2). These data are used to obtain 359 genes that are differentially expressed among DBA/2, C57BL/6, and 129.

2024-06-30 | GSE234896 | GEO

mRNA-Seq profiling of Stra8-deficient and wild-type testes

2015-04-03 | GSE67169 | GEO

Proteomic analysis of the effects of DDX20 deletion in spermatogonia

Project description:To investigate the role of DDX20 in spermatogenesis, we generated Ddx20 flox/flox Ddx4-Cre mice to make a germ cell-specific Ddx20 knockout, and isolated spermatogonia from four-day-old mouse testes by THY1 magnetic beads. We then performed proteomic analysis using protein lysates obtained from THY1 + spermatogonia.

2024-04-24 | PXD038170 |

RNA-seq reveals an abnormal transcriptome as a result of DDX20 loss in spermatogonia.

Project description:To investigate the role of DDX20 in spermatogenesis, we generated Ddx20 flox/flox Ddx4-Cre mice to make a germ cell-specific Ddx20 knockout, and isolated spermatogonia from four-day-old mouse testes by THY1 magnetic beads.We then performed gene expression profiling analysis using data obtained from RNA-seq of THY1 + spermatogonia.

2023-10-01 | GSE217685 | GEO

mRNA-Seq profiling of Stra8-deficient and wild-type testes

Project description:We sequenced mRNA in grossly enlarged testes from 1-year-old Stra8-deficient mice, and in testes from adult male wild-type controls, to verify that Stra8-deficient testes are enriched for genes normally expressed in type A spermatogonia. Examination of mRNA levels in 6 whole-testis samples (3 replicates of each genotype). Specifically, we sequenced mRNA in grossly enlarged testes from three 1-year-old Stra8-deficient male mice, and in normal testes from three adult male wild-type controls

2015-04-03 | E-GEOD-67169 | biostudies-arrayexpress

Gene expression in undifferentiated spermatogonia

Project description:RNA-seq of sorted undifferentiated spermatogonia from synchronized postnatal testes.

2020-07-22 | GSE144923 | GEO