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ABSTRACT: Lung issue sequencing data after chromate exposure

PROVIDER: PRJNA989128 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR25080400_1.fastq.gz	Fastqsanger.gz
	SRR25080400_2.fastq.gz	Fastqsanger.gz
	SRR25080401_1.fastq.gz	Fastqsanger.gz
	SRR25080401_2.fastq.gz	Fastqsanger.gz
	SRR25080402_1.fastq.gz	Fastqsanger.gz

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Comparison of gene expression profiles in chromate transformed BEAS-2B cells

Project description:We established chromate transformed cell lines by chronic exposure of normal human bronchial epithelial BEAS-2B cells to low doses of hexavalent chromium followed by anchorage-independent growth. The gene expression profiles were analyzed in the established cell lines. The gene expression profiles from six chromate transformed cell lines were remarkably similar to each other yet differed significantly from that of either control cell line or normal Beas-2B cells. A total of 409 differentially expressed genes were identified in chromate transformed cells compared to control cells.

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Project description:Transcriptional profiling of Arabidopsis thalina seedlings in response to high (140 M-BM-5M) and low (20 M-BM-5M) concentrations of chromate (K2CrO4). Low concentrations of chromate (e.g. 40 M-BM-5M) promoted primary root growth, while high concentrations (e.g. 140 M-BM-5M) repressed growth and increased formation of root hairs, lateral roots and adventitious roots. Three-condition experiment, high (140 M-BM-5M) and low (20 M-BM-5M) concentrations of chromate (K2CrO4) vs non-chromate added (Control). Four biological replicates, one replicate per array.

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Exposure to lung-migrating helminth protects against murine SARS-CoV-2 infection through macrophage-dependent T cell activation

Project description:Helminth infection leads to lung remodelling. We used single cell ATAC sequencing (scRNA-seq) to analyze the changes in lung cells following exposure to N. brasiliensis at day 28 and day 45 post helminth infection

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Exposure of an immortalized human bronchial epithelial cell line, BEAS-2B, to one of four metals (arsenic, chromium, nickel or vanadium) to determine the early changes that lead to cell transformation

Project description:To determine early changes leading to human cell transformation (cancer) we exposed an immortalized human bronchial epithelial cell line, BEAS-2B, to one of four different metals that may cause cancer via inhalation in humans or rodents: 2.0 micro-Molar soluble sodium arsenite (NaAsO2), 0.50 micro-Molar potassium chromate (K2CrO4), 250 micro-Molar nickel (II) sulfate (NiSO4), 10 micro-Molar sodium meta-vanadate (NaVO3), or were left untreated (control). After a 30-60 day exposure, cells were rinsed of metals and seeded in soft agar. A small number of the cells formed colonies in the soft agar, demonstrating the potential for anchorage independent growth, a characteristic of cancer. These colonies that originated from a single cell were extracted from the agar and grown out in monolayer for 3-4 weeks. The RNA data provided here is taken from these cells. The significance it that the metal exposure was stopped many generations before the analysis, yet each sample demonstrates changes in gene expression based on the original metal exposure.

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