Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mtb HLA-E tetramer sorted CD8+ T-cells have a diverse TCR repertoire

ABSTRACT: Mtb HLA-E tetramer sorted CD8+ T-cells have a diverse TCR repertoire

PROVIDER: PRJNA989142 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Mtb HLA-E tetramer sorted CD8+ T-cells have a diverse TCR repertoire

Project description:HLA-E molecules can present self and pathogen-derived peptides to both NK-cells and T-cells. T-cells that recognize HLA-E peptides via their T-cell receptor (TCR) are termed donor-unrestricted T-cells due to restricted allelic variation of HLA-E. The composition and repertoire of HLA-E TCRs is not known so far. We performed TCR sequencing on CD8+ T-cells from 21 individuals recognizing HLA-E tetramers (TM) folded with 2 Mtb HLA-E restricted peptides. We sorted HLA-E Mtb TM+ and TMCD8+ T-cells directly ex vivo and performed bulk RNA-sequencing and single cell TCR sequencing. The identified TCR repertoire was diverse and showed no conservation between and within individuals. TCRs selected from our single cell TCR sequencing data could be activated upon HLA-E/peptide stimulation, although not robust, reflecting potentially weak interactions between HLA-E peptide complexes and TCRs. Thus, HLA-E Mtb specific T-cells have a highly diverse TCR repertoire.

2024-02-04 | GSE236154 | GEO

Single cell RNA and TCR profiling of tetramer sorted mouse mLN and IELs.

Project description:We utilized H2-I-Ab tetramers to isolate CD4 T cells from mice to characterize the functional characteristics of ileal intraepithelial T cells and their lymphoid counterparts in the corresponding draining lymph nodes (mLN). Non-CD4 T cells (B220+, CD11b+, CD11c+, F4/80+, CD8a+) were excluded before FACS-sorting of CD45+CD3+CD4+ tetramer-specific T cells discriminated by double-positive staining with dual-labeled tetramer fluorophores. We recovered tetramer+ and tetramer- IELs and mLN T cells, and profiled them by single-cell RNA and TCR sequencing.

2022-09-01 | GSE196426 | GEO

Sorted Bulk and KK10-specific CD8+ T cells from HLA-B*2705 HIV chronic progressors before and after KK10 peptide stimulation

Project description:CD8+ T cells from HLA-B*2705 HIV+ chronic progressors Hi and Lo samples are CD8+ T cells bound by an HLA-B*2705 tetramer specific for the Gag p24 peptide KK10

2014-04-15 | GSE56775 | GEO

Sorted Bulk and KK10-specific CD8+ T cells from HLA-B*2705 HIV elite controllers before and after KK10 peptide stimulation

Project description:CD8+ T cells from HLA-B*2705 HIV+ elite controllers Hi and Lo samples are CD8+ T cells bound by an HLA-B*2705 tetramer specific for the Gag p24 peptide KK10

2014-04-23 | GSE56971 | GEO

HLA-E Presents Glycopeptides from the Mtb Protein MPT32 to Human CD8 T Cells

Project description:Infection with Mycobacterium tuberculosis (Mtb), the bacterium that causes Tuberculosis, remains a global health concern. Both classically and non-classically restricted cytotoxic CD8+ T cells are important to the control of Mtb infection. We and others have demonstrated that the non-classical MHC I molecule HLA-E can present pathogen-derived peptides to CD8+ T cells. In this manuscript, we identified the antigen recognized by an HLA-E-restricted CD8+ T cell clone isolated from an Mtb latently infected individual as a peptide from the Mtb protein, MPT32. Recognition by the CD8+ T cell clone required N-terminal O-linked mannosylation of MPT32 by a mannosyltransferase encoded by the Rv1002c gene. This is the first description of a post-translationally modified Mtb-derived protein antigen presented in the context of an HLA-E specific CD8+ T cell immune response. The identification of an immune response that targets a unique mycobacterial modification is novel and has practical impact in the development of vaccines and diagnostics.

2018-10-26 | PXD005765 | Pride

Sorted Bulk and KK10-specific CD8+ T cells from HLA-B*2705 HIV chronic progressors before and after KK10 peptide stimulation

Project description:CD8+ T cells from HLA-B*2705 HIV+ chronic progressors Hi and Lo samples are CD8+ T cells bound by an HLA-B*2705 tetramer specific for the Gag p24 peptide KK10 PBMCs were incubated in the presence and absence of KK10 peptide for 6 days, and CD8+ and KK10-specific CD8+ T cell were sorted by flow cytometry

2014-04-15 | E-GEOD-56775 | biostudies-arrayexpress

Sorted Bulk and KK10-specific CD8+ T cells from HLA-B*2705 HIV elite controllers before and after KK10 peptide stimulation

Project description:CD8+ T cells from HLA-B*2705 HIV+ elite controllers Hi and Lo samples are CD8+ T cells bound by an HLA-B*2705 tetramer specific for the Gag p24 peptide KK10 PBMCs were incubated in the presence and absence of KK10 peptide for 6 days, and CD8+ and KK10-specific CD8+ T cell were sorted by flow cytometry

2014-04-23 | E-GEOD-56971 | biostudies-arrayexpress

Single cell RNA-seq of human CD8+ T cells binding the IAV HLA-A24/PB1 498-505 tetramer

Project description:PBMCs from 4 donors were stained with the influenza A virus HLA-A24/PB1 498-505 tetramer. Two donors (non-LIFT8 and non-LIFT12) showed TCR independent KIR binding to tetramers whereas the other two donors (Non-LIFT14 and Non-LIFT10) showed TCR specific binding. Cells were single-cell sorted on a BD Aria III into 96-well plates containing 0.5 μl dNTP mix (10 mM), 0.5 μl oligo-dT pri- mer (5 μM), and 1 μl lysis buffer (prepared by adding 1 μl RNase inhibitor to 19 μl Triton X-100 solution, 0.2% v/v).

2021-04-08 | E-MTAB-10109 | biostudies-arrayexpress

HLA association with active TB

Project description:Several HLA allelic variants have been associated with protection from, or susceptibility to infectious and autoimmune diseases. Here, we examined whether specific HLA alleles would be associated with different Mtb infection outcomes. We found that DQA1*03:01, DPB1*04:02, and DRB4*01:01 were signficantly more frequent in inividuals with active TB (susceptibility alleles). Furthermore, individuals who express any of the three susceptibility alleles were associated with lower magnitude of responses against Mtb antigens. We investigated the gene expression changes induced in PBMCs by Mtb lysate and a peptide pool (MTB300) in individuals with or without expression of the susceptibility alleles.

2023-03-29 | GSE207214 | GEO

Single cell RNA and TCR profiling of tetramer sorted mouse mLN and IELs.

Project description:Single cell RNA and TCR profiling of tetramer sorted mouse mLN and IELs.

| PRJNA804878 | ENA